[KO Validated] UBE2C Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00762

Core Product Specifications and Parameters

Biological Background: UBE2C Function and Localization

• UBE2C (Ubiquitin-conjugating enzyme E2 C) is a member of the ubiquitin-conjugating enzyme family, accepting ubiquitin from the E1 complex and catalyzing its covalent attachment to substrate proteins.
• In vitro, UBE2C catalyzes both 'Lys-11'- and 'Lys-48'-linked polyubiquitination, indicating its role in forming different types of ubiquitin chains.
• UBE2C is an essential component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis.
• Within the APC/C, UBE2C initiates 'Lys-11'-linked polyubiquitin chains on substrates, targeting them for proteasomal degradation and promoting mitotic exit.
• Alternative splicing of UBE2C produces multiple isoforms, and the protein undergoes post-translational modifications including acetylation and phosphorylation.
• Keywords associated with UBE2C include cell cycle, cell division, mitosis, and Ubl conjugation pathway, underscoring its central role in cell cycle regulation.

Experimental Guidance and Technical Tips

• This polyclonal antibody was raised against full-length recombinant UBE2C, likely recognizing multiple epitopes for enhanced detection sensitivity.
• It has been validated for knockout (KO) studies, making it suitable for confirming UBE2C depletion in CRISPR/Cas9 or siRNA experiments.
• For Western blot (WB), consider optimizing the antibody dilution using a positive control lysate (e.g., HeLa cells, which express UBE2C). The predicted molecular weight is ~20 kDa.
• For immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization based on the target's association with the APC/C during mitosis.
• For ELISA, perform checkerboard titrations to determine optimal coating and detection conditions.

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CamelBio serves as your one-stop sourcing partner for IVD raw materials, providing high-quality antibodies like this UBE2C polyclonal antibody for cell cycle and cancer research. Beyond this product, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Contact us to streamline your diagnostic development from concept to clinic.