[KO Validated] TTLL12 Rabbit Polyclonal Antibody for WB, ELISA - Q14166

Core Product Specifications and Parameters

Biological Background: TTLL12 Function and Localization

• TTLL12 (Tubulin--tyrosine ligase-like protein 12), also known as dJ526I14.2, is an inactive member of the TTLL family that negatively regulates tubulin post-translational modifications, including detyrosination of the C-terminus and polyglutamylation of glutamate residues. Related references: PMID:20162578, PMID:23251473
• It indirectly promotes histone H4 trimethylation at Lys-20 (H4K20me3), contributing to chromatin structure and epigenetic regulation. Related references: PMID:23251473
• By controlling tubulin and histone H4 modifications, TTLL12 plays a role in mitosis and maintaining chromosome number stability. Related references: PMID:20162578, PMID:23251473
• During RNA virus infection, TTLL12 acts as a negative regulator of the RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE, thus modulating innate immune responses. Related references: PMID:28011935
• TTLL12 localizes to the cytoplasm, midbody, centrosome, spindle, and nucleus, consistent with its roles in cell division and gene regulation.
• It is expressed in the basal layer of prostate and endothelial cells, with increased expression observed in prostatic intraepithelial neoplasia and metastatic lesions, suggesting a potential role in cancer progression.
• As an ATP- and nucleotide-binding protein, TTLL12 is identified through proteomics and is a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (a.a. 485–644), which may confer epitope specificity; consider using full-length protein or peptide competition to confirm binding.
• For Western blot, a band near 74 kDa is expected; validate using positive and negative controls, and consider using KO-validated lysates if available.
• The antibody reacts with human, mouse, and rat samples, making it suitable for cross-species studies in those organisms; always verify reactivity in your model system.
• For ELISA, titration of the antibody and optimization of coating conditions are recommended to achieve optimal signal.
• Since the product is labeled as KO Validated, it may be useful for confirming target knockdown; however, users should perform appropriate validation experiments tailored to their specific experimental design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents, supporting oncology, immunology, and cell biology research. Our TTLL12 antibody, for instance, enables studies of tubulin modification, mitosis, chromosome stability, and viral infection pathways. For custom or rare-target sourcing needs, our expert team assists from discovery to delivery.