[KO Validated] STAT5B Rabbit pAb for WB, IHC-P, IF/ICC, IP, ELISA - P51692

Core Product Specifications and Parameters

Biological Background: STAT5B Function and Localization

• STAT5B is a signal transducer and transcription activator that mediates cellular responses to cytokines, hormones, and growth factors, playing a central role in hematopoietic and erythroid differentiation. Related references: PMID:29844444, PMID:9188471
• It binds to the gamma-activated site (GAS) element in target gene promoters, activating transcription in response to prolactin (PRL) and other stimuli.
• STAT5B participates in the oncostatin-M-mediated signaling pathway through the type II OSM receptor complex (OSMR/IL6ST).
• The protein shuttles between the cytoplasm and nucleus, undergoing nuclear translocation upon activation.
• Key structural features include an SH2 domain for receptor recruitment and dimerization, and it is regulated by phosphorylation.
• Mutations in STAT5B have been associated with dwarfism, reflecting its critical role in growth regulation.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (amino acids 586-787), enabling detection of full-length and potentially certain truncated isoforms.
• In Western blot, the expected molecular weight is approximately 90 kDa; validation in the relevant sample system is recommended.
• This antibody is predicted to react with human, mouse, and rat STAT5B; however, cross-reactivity should be experimentally confirmed for each species and application.
• For immunohistochemistry and immunofluorescence, consider antigen retrieval conditions and include appropriate positive and negative tissue controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with reliable, one-stop access to IVD raw materials—including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. We support signal transduction research by sourcing rare-target reagents such as STAT5B antibodies, helping you accelerate assay development from concept to clinic.