[KO Validated] SMARCC1/BAF155 Rabbit pAb for WB, IP, ELISA - Q92922

Core Product Specifications and Parameters

Biological Background: SMARCC1/BAF155 Function and Localization

• SMARCC1 (also known as BAF155, SWI/SNF complex 155 kDa subunit, HYC5, Rsc8, SRG3, SWI3, CRACC1) is a core subunit of the ATP-dependent SWI/SNF chromatin remodeling complexes.
• Involved in chromatin remodeling: it alters DNA-histone contacts within nucleosomes in an ATP-dependent manner, facilitating transcriptional activation and repression of select genes. May stimulate the ATPase activity of the catalytic subunit of the complex. Related references: PMID:10078207 | PMID:29374058
• Component of the neural progenitor-specific npBAF complex, which is essential for self-renewal and proliferative capacity of multipotent neural stem cells. As neurons exit the cell cycle and differentiate, a switch to the neuron-specific nBAF complex occurs, involving substitution of homologous subunits (e.g., ACTL6A to ACTL6B, PHF10 to DPF1 or DPF3). The nBAF complex, along with CREST, regulates genes required for dendrite growth.
• Localizes to both the nucleus and the cytoplasm.
• Widely expressed across tissues, including brain, heart, muscle, placenta, lung, liver, kidney, and pancreas.
• Post-translationally modified by acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond). These modifications may regulate its assembly into complexes or chromatin targeting.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 80–320 of human SMARCC1, a region that may be exposed and antigenic; verify specificity on endogenous protein in relevant cell or tissue lysates.
• For WB, consider the predicted molecular weight of ~123 kDa; use reducing conditions and appropriate molecular weight markers. Validate with a loading control and, if available, KO cell lines.
• When performing IP, optimize antibody-to-lysate ratios and include appropriate isotype controls. Crosslink the antibody to beads if heavy/light chain interference is a concern.
• For ELISA, determine optimal coating antigen concentration and antibody dilution through checkerboard titration. Include blocking steps and appropriate blanks.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with streamlined access to premium IVD raw materials. This anti-SMARCC1/BAF155 polyclonal antibody exemplifies our commitment to supporting chromatin biology and neurodevelopment studies. Beyond single antibodies, we supply validated antibody pairs, optimized monoclonal and polyclonal reagents, bulk ancillary materials, and custom sourcing for hard-to-find targets—bridging the gap from concept to clinic.