[KO Validated] RPL22 Rabbit pAb - P35268

Core Product Specifications and Parameters

Biological Background: RPL22 Function and Localization

• RPL22 encodes the 60S ribosomal protein L22 (eL22), a component of the large ribosomal subunit, and the ribosome is responsible for cellular protein synthesis. Related references: PMID:23636399 PMID:32669547
• Alternative names include EBER-associated protein (EAP), Epstein-Barr virus small RNA-associated protein, and heparin-binding protein HBp15.
• It localizes primarily in the cytoplasm, where ribosome assembly and translation take place.
• The protein is classified as a heparin-binding, phosphoprotein, and RNA-binding ribosomal protein.
• It is part of the large ribosomal subunit protein family and has been identified by direct protein sequencing and proteomics.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-122) of human RPL22, which may allow detection of both full-length and partially cleaved protein forms.
• For Western blotting, a band at approximately 15 kDa is expected; consider using a 12-15% SDS-PAGE gel for optimal resolution.
• In immunofluorescence/immunocytochemistry (IF/ICC), confirm the cytoplasmic localization of RPL22 in your cell line of interest, and validate antibody specificity using appropriate controls.
• For ELISA, the antibody can be used for detecting recombinant RPL22 protein; titration is recommended for optimal signal-to-noise ratio.
• Cross-reactivity with mouse and rat suggests high sequence conservation; however, always validate in the relevant sample system to confirm binding performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs and optimized polyclonal antibodies like this anti-RPL22 antibody, to support ribosomal protein and translation-related studies. We offer bulk ancillary reagents and expert sourcing for rare targets, streamlining your development from concept to clinic.