[KO Validated] RAG2 Rabbit pAb for WB, ELISA - P55895

Core Product Specifications and Parameters

Biological Background: RAG2 Function and Localization

• Core component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination, assembling a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B- and T-lymphocytes.
• RAG2 is required for all known catalytic activities mediated by RAG1 within the RAG complex, likely acting as a sensor of chromatin state that recruits the complex to histone H3 trimethylated at Lys-4 (H3K4me3).
• V(D)J recombination involves a two-step DNA cleavage mechanism: initial nicking upstream of the heptamer, followed by a direct transesterification reaction generating hairpin coding ends and blunt 5'-phosphorylated signal ends.
• The RAG complex also plays a role in pre-B cell allelic exclusion, enforcing clonality and monospecific B-cell antigen receptor expression by repositioning the second immunoglobulin allele to pericentromeric heterochromatin.
• Localizes exclusively to the nucleus, consistent with its role in DNA recombination and chromatin interaction.
• Expressed specifically in cells of the B- and T-lymphocyte lineages.
• Contains zinc-finger domains and binds metal ions; classified as a chromatin regulator involved in DNA recombination and associated with severe combined immunodeficiency (SCID).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 351-527 of human RAG2; this region may confer reactivity across species but should be validated in your target sample, especially if using non-human/mouse systems.
• For Western blotting, consider using nuclear extracts from B- or T-cell lines (e.g., Jurkat, Raji) as positive controls, with a predicted molecular weight of approximately 59 kDa.
• ELISA applications may require optimization of coating antigen and detection conditions; the antibody's polyclonal nature can provide robust signal but may also exhibit background in complex samples.
• Since RAG2 expression is restricted to developing lymphocytes, ensure your experimental system contains the appropriate cell populations; induced or primary cells may require careful handling.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This RAG2 polyclonal antibody supports research into V(D)J recombination and immune development; CamelBio’s supply network can source validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare target raw materials to streamline your assay development.