[KO Validated] PPP1CC Rabbit pAb - P36873

Core Product Specifications and Parameters

Biological Background: PPP1CC Function and Localization

• Encodes the catalytic subunit of protein phosphatase 1 (PP1) that associates with over 200 regulatory proteins to form substrate-specific holoenzymes, dephosphorylating hundreds of targets to regulate numerous cellular processes (PubMed:17936702, PubMed:25012651). Related references: PMID:17936702 PMID:25012651
• Essential for cell division; participates in the regulation of glycogen metabolism, muscle contractility, and protein synthesis (PubMed:17936702). Related references: PMID:17936702
• Component of the PTW/PP1 phosphatase complex, controlling chromatin structure and cell cycle progression during mitosis-to-interphase transition (PubMed:20516061). Related references: PMID:20516061
• In balance with CSNK1D and CSNK1E, determines circadian period length by regulating the speed and rhythmicity of PER1 and PER2 phosphorylation (PubMed:21712997). Related references: PMID:21712997
• Regulates the recruitment of the SKA complex to kinetochores, ensuring proper chromosome segregation (PubMed:28982702). Related references: PMID:28982702
• Dephosphorylates FOXP3 at Ser-418 in regulatory T-cells from rheumatoid arthritis patients, inactivating FOXP3 and contributing to Treg dysfunction (PubMed:23396208). Related references: PMID:23396208
• Along with PPP1CA, dephosphorylates and activates the innate immune sensors IFIH1/MDA5 and RIG-I (PubMed:23499489). Related references: PMID:23499489
• Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that dephosphorylates and activates RAF family kinases, thereby promoting MAPK pathway signaling (PubMed:35768504, PubMed:35831509). Related references: PMID:35768504 PMID:35831509
• Dephosphorylates MKI67 at the onset of anaphase (PubMed:25012651). Related references: PMID:25012651
• Localizes to multiple subcellular compartments including the cytoplasm, nucleus, nucleolus, centrosome, kinetochores, and midbody, reflecting its diverse functional roles.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight of PPP1CC is approximately 37 kDa; consider using 10% SDS-PAGE and appropriate transfer conditions, and include proper controls.
• For immunofluorescence/immunocytochemistry, fixation and permeabilization methods may need optimization; validate in relevant cell lines endogenously expressing PPP1CC.
• Suitable for ELISA; for sandwich ELISA, consider pairing with a validated matched antibody pair and optimize coating and detection conditions.
• Given the high sequence conservation among human, mouse, and rat, cross-reactivity is expected; always verify specificity in your experimental model using appropriate knockdown or knockout controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to validated IVD raw materials, including high-quality anti-PPP1CC polyclonal antibodies for studying phosphatase signaling, cell cycle regulation, and MAPK pathway dynamics. As a one-stop sourcing partner, we provide optimized antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets—accelerating your assay development from concept to clinic.