[KO Validated] p53 Rabbit pAb for WB, IHC-P, IF/ICC, IP, ELISA - P04637

Core Product Specifications and Parameters

Biological Background: p53 (TP53) Function and Localization

• Multifunctional transcription factor that induces cell cycle arrest, DNA repair or apoptosis, acting as a tumor suppressor in many tumor types (PubMed:11025664, PubMed:12524540, PubMed:12810724). Related references: PMID:11025664, PMID:12524540, PMID:12810724
• Apoptosis induction is mediated by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression (PubMed:12524540, PubMed:17189187). Related references: PMID:12524540, PMID:17189187
• Pro-apoptotic activity is activated via interaction with ASPP1 or ASPP2 and inhibited by iASPP (PubMed:12524540). Related references: PMID:12524540
• In cooperation with mitochondrial PPIF, p53 activates oxidative stress-induced necrosis, largely independent of transcription.
• Regulates the circadian clock by repressing CLOCK-BMAL1-mediated transcriptional activation of PER2 (PubMed:24051492). Related references: PMID:24051492
• Subcellular localization includes cytoplasm, nucleus, PML body, endoplasmic reticulum, mitochondrion matrix, and centrosome.
• Multiple isoforms are expressed in a tissue-dependent manner, with isoform 2 enhancing transactivation, isoform 4 suppressing growth suppression, and isoform 7 inhibiting apoptosis.

Experimental Guidance and Technical Tips

• For WB, consider using a dilution series to optimize detection of the 44kDa p53 band.
• The immunogen spans amino acids 1-393 of human p53; validate the antibody in your relevant sample system.
• For IHC-P, antigen retrieval may be required; test in positive control tissues such as tumor samples.
• As a KO Validated antibody, use p53 knockout cell lysates as a negative control to confirm specificity.
• In IP experiments, ensure appropriate antibody concentration to capture p53 complexes.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For research in tumor biology, apoptosis, and cell cycle regulation, we supply rare target raw materials like this p53 antibody to accelerate diagnostic development. Contact us for customized procurement solutions tailored to your project requirements.