[KO Validated] NSD3/WHSC1L1 Rabbit pAb for WB, ELISA - Q9BZ95

Core Product Specifications and Parameters

Biological Background: NSD3/WHSC1L1 Function and Localization

• NSD3 (also designated WHSC1L1, KMT3F, KMT3G, WHISTLE) is a histone-lysine N-methyltransferase belonging to the NSD protein family.
• It preferentially dimethylates histone H3 at Lys-4 (H3K4me2) and Lys-27 (H3K27me2), serving as an epigenetic regulator that can mark transcriptional activation or repression, respectively.
• Localized predominantly to the nucleus and associates with chromosomes, reflecting its role in chromatin modification.
• NSD3 is highly expressed in brain, heart, and skeletal muscle, with lower levels in liver and lung.
• The protein undergoes multiple post-translational modifications, including acetylation, phosphorylation, and ubiquitination; it contains zinc-finger domains and a catalytic SET domain.
• NSD3 is classified as a proto-oncogene and is involved in chromosomal rearrangements, linking it to cancer development.
• Alternative splicing of the NSD3 gene generates several isoforms with potential functional diversity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 400–600 of human NSD3/WHSC1L1, a region likely conserved across species, which supports the reported cross-reactivity with mouse and rat; still, verify in your specific sample context.
• For Western blotting, NSD3 is a large protein (~162 kDa); consider using a high-molecular-weight marker and optimizing transfer conditions (e.g., extended transfer time, low methanol) to ensure efficient detection.
• This polyclonal antibody has been KO validated; confirm specificity in your laboratory using knockout cell lines or tissue samples.
• In ELISA applications, coat with recombinant NSD3 protein or peptide; titration of both coating antigen and antibody may be necessary to achieve optimal signal-to-noise ratios.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for diagnostic manufacturers and research laboratories, offering a single-point supply of validated IVD raw materials. This NSD3/WHSC1L1 polyclonal antibody supports epigenetic and oncology research, and we provide an extensive portfolio of antibodies, matched antibody pairs, and bulk ancillary reagents. For rare or custom targets, our sourcing capabilities can help bridge your development needs from early R&D to clinical application.