[KO Validated] NRF2 Rabbit Monoclonal Antibody - Q16236

Core Product Specifications and Parameters

Biological Background: NRF2 Function and Localization

• NRF2 is a transcription factor that binds to antioxidant response elements (ARE) in the promoters of cytoprotective genes, such as phase 2 detoxifying enzymes, and promotes their expression to neutralize reactive electrophiles. Related references: PMID:11035812, PMID:19489739, PMID:29018201
• Under basal conditions, NRF2 is ubiquitinated and degraded in the cytoplasm by the KEAP1 complex; upon oxidative stress, electrophile metabolites inhibit KEAP1, allowing NRF2 nuclear accumulation, heterodimerization with small Maf proteins, and ARE‑driven gene activation. Related references: PMID:11035812, PMID:15601839, PMID:19489739, PMID:29018201, PMID:29590092
• The NRF2 pathway is also activated by selective autophagy (via KEAP1‑SQSTM1/p62 interaction) and by the unfolded protein response, contributing to redox homeostasis and cell survival following endoplasmic reticulum stress. Related references: PMID:20452972
• NRF2 may be involved in transcriptional activation of the β‑globin gene cluster by mediating enhancer activity of hypersensitive site 2 of the β‑globin locus control region. Related references: PMID:7937919
• NRF2 is a critical regulator of the innate immune response: it suppresses macrophage inflammatory cytokine transcription, represses antiviral cytosolic DNA sensing by downregulating STING1, and limits pro‑inflammatory cytokine release during SARS‑CoV‑2 infection while inhibiting replication of diverse pathogenic viruses through an interferon‑independent mechanism. Related references: PMID:30158636, PMID:33009401
• Subcellular localization is primarily cytoplasmic under unstressed conditions, with nuclear translocation upon oxidative or electrophilic stress; it is widely expressed, with highest levels in adult muscle, kidney, lung, liver, and fetal muscle.
• Post‑translational modifications include acetylation, glycation, phosphorylation, and ubiquitination, which modulate its stability, activity, and interactions.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 243‑400 of human NRF2, a region that includes the DNA‑binding domain and part of the Neh1 domain; consider this when designing epitope‑specific assays.
• For Western blot, detect a band at approximately 68 kDa; validate the signal using knockout cell lysates if available to confirm specificity.
• For ChIP applications, optimize cross‑linking and sonication conditions for your cell type, and include appropriate positive/negative control regions.
• In ELISA, pair this antibody with a suitable detection antibody after confirming reactivity with your target antigen format.
• Although validated for human, mouse, and rat, verify reactivity in your specific experimental species and tissue type before scaling up.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering diagnostic manufacturers and researchers streamlined access to high‑quality antibodies, including this KO‑validated anti‑NRF2 rabbit monoclonal antibody. With expertise spanning oxidative stress and inflammatory research, CamelBio provides validated antibody pairs, bulk ancillary reagents, and rare‑target raw‑material sourcing to accelerate your development from concept to clinic.