[KO Validated] NRF2 Rabbit Monoclonal Antibody for WB, IP, ELISA, ChIP - Q16236

Core Product Specifications and Parameters

Biological Background: NRF2 Function and Localization

• NRF2 (Nuclear factor erythroid 2-related factor 2), encoded by the NFE2L2 gene, is a transcription factor belonging to the cap'n'collar family. It is also known as HEBP1, Nrf-2, IMDDHH, or F2.
• Binds to antioxidant response elements (ARE) in promoters of cytoprotective genes, including phase 2 detoxifying enzymes, to neutralize reactive electrophiles. Related references: PMID:11035812, PMID:19489739, PMID:29018201
• Under basal conditions, NRF2 is ubiquitinated and degraded by the KEAP1 complex; oxidative stress inhibits KEAP1, promoting NRF2 nuclear accumulation, heterodimerization with small Maf proteins, and ARE binding. Related references: PMID:11035812, PMID:15601839, PMID:29018201, PMID:19489739, PMID:29590092
• Selective autophagy activates NRF2 through SQSTM1/p62 interaction with KEAP1, leading to cytoprotective gene expression. Related references: PMID:20452972
• May regulate beta-globin cluster transcription by mediating hypersensitivity site 2 enhancer activity. Related references: PMID:7937919
• Represses antiviral cytosolic DNA sensing by suppressing STING1 expression, and inhibits replication of viruses including SARS-CoV-2, HSV-1, HSV-2, Vaccinia, and Zika through a type I interferon-independent mechanism. Related references: PMID:30158636, PMID:33009401
• Subcellular localization is predominantly cytoplasmic under normal conditions, but translocates to the nucleus upon activation. Widely expressed, with highest levels in adult muscle, kidney, lung, liver, and fetal muscle.
• Undergoes post-translational modifications such as acetylation, phosphorylation, and glycation, and contains a DNA-binding domain.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 505–605 of human NRF2, targeting the C-terminal region. Consider this when selecting detection reagents and validation strategies.
• Expected protein weight is 68 kDa; for WB, use reducing conditions and appropriate positive controls (e.g., human cell lines or tissues expressing NRF2). Verify band specificity by knockout or knockdown validation if possible.
• The antibody is indicated for WB, IP, ELISA, and ChIP. Each application may require individual optimization of experimental conditions (e.g., fixation for ChIP, lysate preparation for IP).
• Cross-reactivity is confirmed for human; performance in other species has not been tested and should be validated in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to premium IVD raw materials, exemplified by this anti-NRF2 monoclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonals, and bulk ancillary reagents for oxidative stress and immunology research. Whether you need a specific clone like this NRF2 antibody or rare-target sourcing, CamelBio is your reliable partner from concept to clinic.