[KO Validated] NCOA2 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q15596

Core Product Specifications and Parameters

Biological Background: NCOA2 Function and Localization

• NCOA2 (Nuclear receptor coactivator 2) is a transcriptional coactivator for steroid and nuclear receptors, specifically coactivating the AF-2 domain but not the AF-1 domain.
  Related references: PMID:23508108, PMID:8670870, PMID:9430642
• Required with NCOA1 to control energy balance between white and brown adipose tissues.
  Related references: PMID:23508108, PMID:8670870, PMID:9430642
• Critical regulator of glucose metabolism; acts as a RORA coactivator to specifically modulate G6PC1 expression.
  Related references: PMID:23508108, PMID:8670870, PMID:9430642
• Involved in positive regulation of glucocorticoid receptor NR3C1 activity via the sumoylation enhancer RWDD3.
  Related references: PMID:23508108
• Positively regulates the circadian clock by acting as a transcriptional coactivator for the CLOCK-BMAL1 heterodimer. (By similarity)
• Subcellular localization: Nucleus.
• Post-translational modifications include acetylation, methylation, phosphorylation, and ubiquitination (isopeptide bond and Ubl conjugation), as indicated by UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 550-820 of human NCOA2. For cross-reactivity with rat samples, alignment of this region with rat NCOA2 should be verified; the antibody is reported to cross-react with rat.
• For Western blotting, the predicted molecular weight is ~159 kDa; use an appropriate gel percentage (e.g., 6-8% SDS-PAGE) and optimize transfer conditions to ensure efficient blotting of large proteins.
• For immunohistochemistry (IHC-P), suitable antigen retrieval methods and positive control tissues expressing NCOA2 should be included; nuclear staining is expected.
• In ELISA, the antibody can be used as a capture or detection reagent; titrate against recombinant NCOA2 protein for optimal sensitivity.
• As a polyclonal antibody, batch-to-batch consistency should be validated for critical experiments, and re-validation may be necessary when switching lots.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop sourcing platform for IVD raw materials, connecting diagnostic manufacturers, labs, and research institutes with a wide range of validated antibodies and related reagents. This anti-NCOA2 rabbit polyclonal antibody supports research on nuclear receptor signaling, metabolic regulation, and circadian biology. In addition to individual antibodies, CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal preparations, bulk ancillary reagents, and assist with rare target raw-material procurement to streamline your projects from discovery to clinic.