[KO Validated] MEK1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q02750

Core Product Specifications and Parameters

Biological Background: MEK1 Function and Localization

• Dual specificity mitogen-activated protein kinase kinase 1 (MAP2K1), also known as MEK1 or MAPKK1, is a key component of the MAPK/ERK signaling cascade.
• It is activated by RAF kinases downstream of RAS, and subsequently phosphorylates both threonine and tyrosine residues in the Thr-Glu-Tyr motif of ERK1 and ERK2, leading to their activation.
• MAP2K1 also activates BRAF in a KSR1 or KSR2-dependent manner by releasing inhibitory intramolecular interactions and promoting KSR1/KSR2-BRAF dimerization (PubMed:29433126). Related references: PMID:29433126
• The MAPK/ERK pathway mediates diverse cellular processes including growth, adhesion, survival, differentiation, and regulation of transcription, metabolism, and cytoskeletal rearrangements.
• MAP2K1 localizes to multiple subcellular compartments including cytoplasm, cytoskeleton (centrosome and spindle pole body), nucleus, and membrane.
• It is widely expressed in tissues, with extremely low levels in the brain.
• Post-translational modifications include phosphorylation, acetylation, and alternative splicing; mutations are associated with cardiomyopathies, ectodermal dysplasia, and intellectual disability (as indicated by UniProt keywords).

Experimental Guidance and Technical Tips

• The immunogen (a synthetic peptide within amino acids 1-100 of human MEK1) suggests the antibody recognizes the N-terminal region; consider this when interpreting results in isoforms or post-translationally modified forms.
• For WB, the predicted molecular weight is 43 kDa; validate band size in your sample system and consider potential shifts due to phosphorylation.
• For IHC-P, note that MEK1 is widely expressed; include appropriate positive and negative control tissues, and validate with knockout models if possible.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize antibody concentration and pairing for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Anti-MEK1 antibodies like this one support research and assay development for the MAPK signaling pathway, which is implicated in cancer and other diseases. CamelBio can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, ancillary reagents, and rare target raw-material sourcing to accelerate your diagnostic development.