[KO Validated] LAMP2 Rabbit PolymAb® - P13473

Core Product Specifications and Parameters

Biological Background: LAMP2 Function and Localization

• LAMP2 is a lysosomal membrane glycoprotein essential for lysosome biogenesis, pH regulation, and autophagy. It directly inhibits TMEM175 to maintain lysosomal acidification. Related references: PMID:11082038, PMID:18644871, PMID:24880125, PMID:37390818

• It functions as a receptor in chaperone-mediated autophagy, binding substrate proteins (e.g., GAPDH, GPX4, NLRP3) via interaction with chaperones like HSPA8 and targeting them for lysosomal degradation. Related references: PMID:11082038, PMID:18644871, PMID:24880125, PMID:36586411

• LAMP2 is required for autophagosome-lysosome fusion during macroautophagy, facilitating the recruitment of STX17 to autophagosomes. Related references: PMID:27628032

• It contributes to MHC class II-mediated presentation of exogenous antigens by supporting lysosomal protein degradation and peptide loading. Related references: PMID:15894275, PMID:20518820

• LAMP2 localizes to the lysosome membrane, endosome membrane, cell membrane, and autophagosome membrane.

• Three main isoforms (LAMP-2A, LAMP-2B, LAMP-2C) exhibit tissue-specific expression; LAMP-2A is highly expressed in placenta, lung, and liver, while LAMP-2B and LAMP-2C show broader distributions. Related references: PMID:26856698, PMID:7488019

• Keywords highlight its involvement in autophagy, glycogen storage disease (disease variant).

• LAMP2 is post-translationally modified by glycosylation and contains disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 29-375 of human LAMP2 (NP_002285.1), corresponding to the lumenal domain, ensuring recognition of the native protein.
• For Western blotting, LAMP2 typically migrates as a diffuse band between 90-120 kDa due to heavy glycosylation, though the calculated molecular weight is approximately 45 kDa. Validate with appropriate positive controls (e.g., HeLa, HEK293 cell lysates) and consider using lysosomal-enriched fractions.
• In immunofluorescence, expect punctate cytoplasmic staining consistent with lysosomal localization; co-staining with lysosomal markers (e.g., LAMP1) is recommended.
• For immunohistochemistry, tissue-specific expression should be validated in the target tissue; antigen retrieval may be required for formalin-fixed paraffin-embedded sections.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This LAMP2 antibody supports autophagy and lysosomal function research, and can be integrated into larger diagnostic or research workflows. Our supply capabilities extend to custom sourcing of rare targets, helping you accelerate from concept to clinic.