[KO Validated] JAK2 Rabbit mAb for WB, IP, IF/ICC, IHC-P, ELISA - O60674

Core Product Specifications and Parameters

Biological Background: JAK2 Function and Localization

• Tyrosine-protein kinase JAK2 (Janus kinase 2) is a non-receptor tyrosine kinase critically involved in signaling by many cytokine receptors, including those for interleukins, interferons, and hormones.
• Upon ligand engagement, JAK2 phosphorylates specific tyrosine residues on the receptor cytoplasmic tails, creating docking sites for STAT proteins, which are then phosphorylated, dimerize, and translocate to the nucleus to activate target gene transcription. Related references: PMID:15690087, PMID:9618263
• In erythropoiesis, erythropoietin (EPO) stimulation triggers JAK2 autophosphorylation and association with the EPOR, leading to STAT5 phosphorylation and activation of essential erythropoietic genes. Related references: PMID:9657743
• JAK2 functions in the nucleus by phosphorylating histone H3 at Tyr-41 (H3Y41ph), which displaces HP1α from chromatin and modulates chromatin structure and gene expression. Related references: PMID:19783980
• It also mediates angiotensin-2-induced ARHGEF1 phosphorylation, CDKN1B phosphorylation in cell cycle control, and retinol-induced STAT5 activation. Related references: PMID:20098430, PMID:21423214, PMID:21368206
• JAK2 is ubiquitously expressed across most tissues and localizes to the cytoplasm, nucleus, and endomembrane system, consistent with its multiple signaling roles.
• JAK2 is annotated as a proto-oncogene with documented chromosomal rearrangements and disease-associated variants; mutations in JAK2 are linked to hematological malignancies.

Experimental Guidance and Technical Tips

• The immunogen (residues 933‑1132) covers the C‑terminal kinase (JH1) and pseudokinase (JH2) domains; consider domain‑specific recognition when using truncation mutants or isoform‑specific assays.
• This antibody is reported for WB, IP, IF/ICC, IHC‑P, and ELISA. In WB, a band at ~131 kDa is expected; validate appropriate loading controls and membrane blocking conditions.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), optimize fixation, antigen retrieval, and permeabilization protocols for your specific tissue or cell type.
• KO validation confirms specificity in WB; always verify reactivity in the relevant species (human, mouse, rat) and sample system before large‑scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers, clinical laboratories, and research institutes to deliver a comprehensive one-stop IVD raw-material sourcing experience. This anti-JAK2 monoclonal antibody supports studies in cytokine signaling, hematopoiesis, and oncogenic kinase pathways. Beyond individual antibodies, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare‑target raw‑material procurement to accelerate your assay development.