[KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb - P17301

Core Product Specifications and Parameters

Biological Background: Integrin alpha-2 Function and Localization

• Protein identity: Integrin alpha-2 (ITGA2), also known as CD49 antigen-like family member B, collagen receptor, GPIa, and VLA-2 subunit alpha, is the alpha subunit of the integrin alpha2/beta1 heterodimer.
• Core function: Serves as a receptor for laminin, collagen, collagen C-propeptides, fibronectin, and E-cadherin, and recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is critical for cell adhesion to collagens, force generation, and extracellular matrix organization.
• Viral receptor: Integrin alpha2/beta1 acts as a host cell receptor for Human rotavirus A and Human echoviruses 1 and 8, facilitating viral entry.
• Subcellular localization: Localizes to the plasma membrane as a single-pass type I transmembrane protein.
• Post-translational features: Glycoprotein with multiple disulfide bonds and metal-ion binding sites for calcium and magnesium, essential for integrin activation and ligand binding.
• Structural domains: Contains integrin alpha repeats, a signal peptide, a long extracellular region, a transmembrane helix, and a short cytoplasmic tail. The immunogen sequence lies within the C-terminal cytoplasmic region (aa 1082-1181).
• Gene family: Belongs to the integrin alpha chain family, which mediates cell-cell and cell-matrix interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal cytoplasmic tail (aa 1082-1181), enabling detection of full-length ITGA2 without interference from potential extracellular domain shedding.
• In western blotting, a specific band at approximately 129 kDa is expected. Use an appropriate loading control and validate in the relevant sample system.
• For immunohistochemistry (IHC-P), optimize antigen retrieval methods and antibody dilutions for your tissue type; consider including knockout control sections to confirm specificity.
• The reported cross-reactivity with mouse and rat supports translational studies; however, we recommend confirming reactivity in your own experimental model.
• This antibody has been used in ELISA; suitable for plate-based binding assays or sandwich ELISA development with paired antibodies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a streamlined, one-stop supply channel for IVD raw materials, connecting diagnostic developers with validated antibodies, antibody pairs, and bulk ancillary reagents. For research involving integrin alpha-2, collagen receptors, or viral entry mechanisms, this [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit mAb is a reliable choice. Our portfolio also includes customized monoclonal/polyclonal antibodies, rare target sourcing, and technical consultation to support your project from concept to clinical application.