[KO Validated] Integrin alpha 2 (ITGA2/CD49b) Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P17301

Core Product Specifications and Parameters

Biological Background: Integrin alpha-2 Function and Localization

• Integrin alpha-2 (ITGA2, CD49b) is a transmembrane glycoprotein that pairs with integrin beta-1 (ITGB1) to form the VLA-2 receptor, a major collagen and laminin receptor on platelets and other cell types.
• It recognizes the proline-hydroxylated G-F-P-G-E-R sequence in collagen and mediates cell adhesion to collagens, laminin, fibronectin, and E-cadherin, facilitating force generation and extracellular matrix organization.
• In hemostasis, ITGA2/ITGB1 is essential for platelet adhesion to subendothelial collagens, modulating thrombus formation and platelet activation.
• Beyond hemostasis, the alpha-2 integrin regulates collagenase gene expression and plays a role in tissue remodeling and wound healing.
• The protein serves as a receptor for human rotavirus A and echoviruses 1 and 8, highlighting its role in host–pathogen interactions.
• ITGA2 is localized to the plasma membrane and contains characteristic integrin structural features, including multiple FG-GAP repeats, metal ion-binding sites, and disulfide bonds, as inferred from its inclusion in the integrin alpha chain family.
• UniProt keywords highlight its involvement in cell adhesion, host-virus interaction, calcium/magnesium binding, and signal transduction (P17301).

Experimental Guidance and Technical Tips

• The immunogen for this polyclonal antibody corresponds to the extracellular region (aa 30–162) of human ITGA2, which includes part of the beta-propeller domain critical for ligand binding; consider sequence alignment with mouse (UniProt Q62469) and rat (Q63223) ITGA2 to predict cross-reactivity.
• For Western blot, expect a band at ~130 kDa; KO validation confirms specificity, but it is advisable to include positive and negative controls in your experimental setup.
• In IHC-P and IF/ICC, optimize antigen retrieval and permeabilization conditions, as the target is a transmembrane protein; fixation methods may affect epitope accessibility.
• For ELISA, use the antibody as a capture or detection reagent after confirming reactivity with native or recombinant ITGA2.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers research-use-only IVD raw materials, including validated primary antibodies, recombinant proteins, and ancillary reagents for cell adhesion and virology research. This integrin alpha-2 antibody exemplifies our range of polyclonal and monoclonal antibodies designed for applications from Western blot to immunoassays. For bulk orders, custom conjugations, or sourcing rare target reagents, CamelBio provides a streamlined procurement bridge from concept to clinic.