[KO Validated] HSP27/HSPB1 Rabbit pAb for WB, IF/ICC, ELISA - P04792

Core Product Specifications and Parameters

Biological Background: HSP27/HSPB1 Function and Localization

• Heat shock protein beta-1 (HSPB1), also known as HSP27 or SRP27, is a small heat shock protein that functions as an ATP-independent molecular chaperone, maintaining denatured proteins in a folding-competent state and preventing aggregation PMID:10383393 PMID:20178975. Related references: PMID:10383393 PMID:20178975

• It plays a key role in stress resistance and actin cytoskeleton organization, contributing to cell stability and motility under stress conditions PMID:19166925. Related references: PMID:19166925

• Through its chaperone activity, HSPB1 regulates the phosphorylation and axonal transport of neurofilament proteins, which is critical for neuronal function PMID:23728742. Related references: PMID:23728742

• The protein localizes to the cytoplasm, nucleus, and associates with the cytoskeleton, including the mitotic spindle, reflecting its dynamic role in cellular architecture and division.

• HSPB1 is ubiquitously expressed with highest levels in cardiac, skeletal, and smooth muscle tissues, consistent with its protective function in mechanically active cells.

• Post-translational modifications include phosphorylation, acetylation, and methylation, which modulate its chaperone activity, oligomerization, and interaction with client proteins.

• Mutations in HSPB1 are linked to Charcot-Marie-Tooth disease type 2F and distal hereditary motor neuropathy, underscoring its importance in peripheral nerve health.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-120) of human HSPB1, suggesting the antibody may recognize epitopes within this domain; consider this when designing peptide blocking experiments or comparing with antibodies targeting other regions.

• Knockout validation confirms specificity; for Western blot, use validated positive and negative cell lysates to ensure reliable detection at the expected 23 kDa band.

• In immunofluorescence/immunocytochemistry, fixation and permeabilization conditions should be optimized, as HSPB1's association with the cytoskeleton may require specific buffers to preserve structure.

• For ELISA, titrate the antibody and ensure appropriate antigen coating; the recombinant protein used as immunogen can serve as a positive control.

• Cross-reactivity with human HSPB1 is reported; reactivity with other species or homologs such as Hsp25 in mouse is not specified and should be experimentally validated if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive partner for IVD raw material procurement, bridging diagnostic manufacturers with high-quality reagents from concept to commercialization. This anti-HSP27/HSPB1 antibody supports research into cellular stress responses, chaperone biology, and neurodegenerative diseases, and can be complemented by CamelBio's portfolio of validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. Contact our team to streamline your supply chain and accelerate assay development with reliable raw materials.