[KO Validated] HNRNPF Rabbit pAb for WB, IF/ICC, ELISA - P52597

Core Product Specifications and Parameters

Biological Background: HNRNPF Function and Localization

• HNRNPF is a component of heterogeneous nuclear ribonucleoprotein (hnRNP) complexes, which facilitate pre-mRNA processing events including splicing and maturation, ensuring the production of functional translatable mRNAs in the cytoplasm.
• It plays a crucial role in the regulation of alternative splicing by binding to G-rich sequences within pre-mRNAs, maintaining target RNAs in an unfolded state to modulate splice site selection.
• The protein is mainly localized in the nucleoplasm, consistent with its role in nuclear RNA processing and spliceosome assembly.
• HNRNPF is subject to various post-translational modifications, including acetylation, phosphorylation, and ubiquitination (isopeptide bond formation), which may dynamically regulate its activity and interactions.
• Belonging to the hnRNP family, it contains RNA-binding motifs (repeats) characteristic of ribonucleoproteins involved in mRNA metabolism.
• It is ubiquitously expressed across human tissues, underscoring its fundamental role in constitutive and alternative splicing regulation.

Experimental Guidance and Technical Tips

• For Western blot, use a standard dilution range for polyclonal antibodies and confirm the expected band at approximately 46 kDa. As a KO-validated antibody, it is particularly useful to verify HNRNPF depletion in knockout cell models.
• In immunofluorescence/immunocytochemistry, nuclear permeabilization and fixation should be optimized to ensure access to the nucleoplasmic target.
• For ELISA, the recombinant immunogen (amino acids 1-280) can serve as a positive control to establish assay sensitivity and linearity.
• Always validate antibody performance in your specific sample matrix, as cross-reactivity profiles may vary under different experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a streamlined procurement channel for IVD manufacturers and researchers seeking validated antibody raw materials. This HNRNPF polyclonal antibody is representative of our extensive catalog, which includes antibody pairs, bulk ancillary reagents, and access to rare-target sourcing. Our support spans the full development cycle, from initial concept to clinical-scale manufacturing, ensuring consistent quality and supply chain reliability.