KO Validated HIF1AN/FIH1 Rabbit mAb for WB, ELISA - Q9NWT6

Core Product Specifications and Parameters

Biological Background: Hypoxia-inducible factor 1-alpha inhibitor (HIF1AN/FIH1) Function and Localization

• HIF1AN, also known as Factor inhibiting HIF-1 (FIH-1), is a dioxygenase that belongs to the oxidoreductase family and requires iron as a cofactor.
• Functions as an oxygen sensor: Under normoxic conditions, it hydroxylates HIF-1 alpha at asparagine 803 in the C-terminal transactivation domain, blocking interaction with transcriptional coactivators like CBP/p300.
• Involved in transcriptional repression through interactions with HIF1A, VHL, and histone deacetylases.
• Also hydroxylates specific asparagine residues within ankyrin repeat domains (ARD) of substrates such as NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A, and others, expanding its regulatory scope.
• Additionally, it hydroxylates aspartate and histidine residues in ARDs of ANK1 and TNKS2, respectively, demonstrating versatile substrate specificity.
• Negatively regulates NOTCH1 activity, thereby accelerating myogenic differentiation.
• Positively regulates ASB4 activity, promoting vascular differentiation.
• Subcellular localization includes the nucleus, cytoplasm, and perinuclear region, consistent with its roles in oxygen sensing and transcriptional regulation.

Experimental Guidance and Technical Tips

• Because the immunogen corresponds to the N-terminal region, the antibody may detect full-length HIF1AN. Validate detection in your specific sample type.
• For Western blot, use reducing conditions and optimize loading amounts; a band near 41 kDa is expected (predicted size may vary due to modifications).
• If performing ELISA, pair with a suitable secondary reagent and titrate antibody concentration for optimal sensitivity.
• The antibody is KO validated, supporting its use for confirming target specificity in genetic perturbation studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal antibodies, and bulk ancillary reagents. This HIF1AN antibody supports research into hypoxia signaling and transcriptional regulation pathways. From concept to clinic, we provide comprehensive raw-material solutions to accelerate your diagnostic development.