[KO Validated] GBP2 Rabbit Polyclonal Antibody for WB and ELISA - P32456

Core Product Specifications and Parameters

Biological Background: GBP2 Function and Localization

• GBP2 (Guanylate-binding protein 2, also known as GBP-2 or HuGBP-2) is an interferon-inducible GTPase that belongs to the guanylate-binding protein family.
• It hydrolyzes GTP to GMP in two consecutive cleavage reactions, with GDP as the major product. Related references: PMID:8706832
• GBP2 plays crucial roles in innate immunity against bacterial, viral, and protozoan pathogens by targeting pathogen-containing vacuoles.
• Upon infection, GBP2 is recruited to vacuoles or escaped bacteria, promoting vacuolar lysis and release of pathogens into the cytosol, which enables detection by inflammasomes.
• It facilitates the release of bacterial ligands like LPS and dsDNA, activating non-canonical CASP4/CASP11 and AIM2 inflammasomes, respectively.
• Independently of its GTPase activity, GBP2 inhibits infectivity of viruses such as HIV-1, Zika, and influenza A by blocking FURIN-mediated maturation of viral envelope proteins. Related references: PMID:31091448
• Subcellularly, GBP2 localizes to cytoplasmic vesicle membranes, Golgi apparatus membrane, cytoplasm, and perinuclear region.
• Post-translational modifications include methylation and prenylation; it is also a lipoprotein and undergoes ubiquitin conjugation.

Experimental Guidance and Technical Tips

• For Western blot, the predicted molecular weight is approximately 67kDa; validate the band in relevant human cell or tissue lysates.
• The immunogen corresponds to amino acids 410–520 in the C-terminal region, making it suitable for detecting full-length GBP2 and some truncated variants.
• Consider using interferon-stimulated samples (e.g., IFN-γ-treated) to enhance endogenous GBP2 expression for detection.
• For ELISA applications, optimize coating and detection conditions; pairing with a capture antibody may require empirical testing.
• Always include appropriate positive and negative controls, and verify specificity with knock-out validated materials when available.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a dedicated partner for diagnostic manufacturers and researchers, providing one-stop access to IVD raw materials, technical services, and consulting from concept to clinic. This anti-GBP2 polyclonal antibody targets a critical innate immunity GTPase, and CamelBio can support your needs with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare target raw materials. If you require tailored solutions for your diagnostic or research project, CamelBio’s integrated supply chain and expertise can help accelerate your development.