[KO Validated] Fatty Acid Synthase (FASN) Rabbit PolymAb® for WB, IF/ICC, ELISA - P49327

Core Product Specifications and Parameters

Biological Background: Fatty Acid Synthase Function and Localization

• Fatty acid synthase (FASN) is a large multifunctional enzyme that catalyzes the de novo biosynthesis of long-chain saturated fatty acids from acetyl-CoA and malonyl-CoA using NADPH.
• The enzyme contains seven distinct catalytic activities and an acyl carrier protein (ACP) domain with a 4'-phosphopantetheine prosthetic group.
• FASN is a key enzyme in lipid metabolism and is ubiquitously expressed, with prominent levels in brain, lung, liver, and mammary gland.
• Subcellularly, FASN localizes to the cytoplasm and has also been detected in melanosomes.
• FASN activity is required for the replication of SARS-CoV-2, indicating a role in viral infection.
• Post-translational modifications include acetylation, phosphorylation, S-nitrosylation, and isopeptide bond formation, which may regulate its function.
• As a member of the short-chain dehydrogenase/reductase superfamily, FASN belongs to the SDR27X1 subfamily (also known as FAS, OA-519).

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 2212–2511 of human FASN, which lies within the terminal region of the enzyme; consider verifying specificity in your assay system.
• For Western blot (WB), a band at approximately 273 kDa is expected; use an appropriate high-molecular-weight gel and transfer conditions.
• In immunofluorescence (IF/ICC), FASN shows a cytoplasmic distribution; optimize permeabilization and fixation protocols for your cell type.
• For ELISA applications, confirm antibody pair compatibility and adjust coating and detection conditions as needed.
• Validate cross-reactivity in mouse and rat samples, as the antibody is predicted to recognize FASN from these species.
• Consider using this antibody to study fatty acid metabolism, cancer metabolism, or viral replication pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by providing validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Our comprehensive portfolio supports metabolic disease and infectious disease research, including fatty acid synthase studies. We assist diagnostic manufacturers and labs in sourcing rare targets and developing reliable assay components, backed by technical consulting from concept to clinic.