[KO Validated] ERK2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P28482

Core Product Specifications and Parameters

Biological Background: ERK2 Function and Localization

• ERK2, encoded by the MAPK1 gene, is also known as mitogen-activated protein kinase 1, extracellular signal-regulated kinase 2, MAP kinase isoform p42, and MAPK2. It is a serine/threonine kinase essential to the MAPK/ERK signal transduction cascade, functioning alongside ERK1 (MAPK3) in response to diverse stimuli.
• ERK2 mediates a wide array of cellular processes including cell growth, adhesion, survival, differentiation, transcription regulation, translation, cytoskeletal rearrangements, and apoptosis through phosphorylation of over 160 identified substrates. These substrates include transcription factors (e.g., ATF2, ELK1, FOS), cytoskeletal elements, apoptosis regulators, and translation regulators. Related references: PMID:35831023
• Beyond its kinase activity, ERK2 acts as a transcriptional repressor that binds to a [GC]AAA[GC] consensus DNA sequence, repressing interferon gamma-induced genes such as CCL5, IRF7, and STAT1. This repressive function is independent of its kinase activity.
• Subcellularly, ERK2 localizes to the cytoplasm, cytoskeleton (including spindle, microtubule organizing center, and centrosome), nucleus, membrane caveolae, and focal adhesions, reflecting its involvement in dynamic cellular processes.
• ERK2 is subject to multiple post-translational modifications, including phosphorylation (critical for its activation), acetylation, and ubiquitin-like conjugation, which regulate its activity, stability, and subcellular localization.
• The kinase specifically phosphorylates PGK1 under hypoxic conditions, directing it to mitochondria and suppressing acetyl-CoA formation from pyruvate. Related references: PMID:26942675

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic peptide spanning amino acids 200–300 of human ERK2 (NP_002736.3). Researchers may consider verifying reactivity in their specific sample system, especially when using non-human, non-mouse, or non-rat models.
• For Western blot (WB) applications, it is recommended to use appropriate positive and negative controls, including knockout-validated lysates, to confirm band specificity at the expected molecular weight (~41 kDa).
• In immunofluorescence/ICC experiments, fixation and permeabilization conditions should be optimized, as ERK2 shuttles between cytoplasm and nucleus depending on stimulation status; using serum starvation and growth factor stimulation may help visualize activation-dependent localization.
• For ELISA, pairing with a suitable capture/detection antibody may enhance sensitivity; users are advised to optimize coating and blocking conditions for their target analyte.
• As a polyclonal reagent, lot-to-lot consistency should be monitored; routine quality control measures are suggested for long-term studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, providing comprehensive access to validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For researchers investigating the MAPK/ERK signaling axis, we offer a portfolio of antibodies targeting key pathway components such as ERK2, along with tailored support for rare-target raw-material procurement. Our integrated supply chain streamlines your transition from concept to clinic, ensuring consistent quality and reliable delivery.