[KO Validated] Claudin 1 Rabbit mAb for WB, ELISA - O95832

Core Product Specifications and Parameters

Biological Background: Claudin-1 Function and Localization

• Claudin-1 is a tight junction integral membrane protein that acts as a major barrier-forming component, regulating paracellular permeability of epithelia. It is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis, essential for normal skin barrier function and water homeostasis (PubMed:23407391) Related references: PMID:23407391
• Claudin-1 acts as a co-receptor for hepatitis C virus (HCV) entry into hepatocytes, associating with CD81 to form the CLDN1-CD81 receptor complex (PubMed:17325668, PubMed:20375010, PubMed:24038151) Related references: PMID:17325668 PMID:20375010 PMID:24038151
• It also serves as a receptor for dengue virus (PubMed:24074594) Related references: PMID:24074594
• Claudin-1 localizes to tight junctions at cell-cell contacts and is present at the cell membrane, predominantly on the basolateral side.
• Strongly expressed in liver and kidney, with additional expression in heart, brain, spleen, lung and testis.
• It belongs to the claudin family, where multiple members are coexpressed and interact to determine overall epithelial permeability.
• Also known as senescence-associated epithelial membrane protein (SEMP1) and contains disulfide bonds.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from residues 112-211, suggesting the antibody may recognize an epitope within this region. Validate specificity in relevant positive tissues such as liver or kidney.
• For Western blot, use appropriate tissue or cell lysates known to express Claudin-1 (e.g., HepG2 for liver) and consider a blocking peptide competition assay to confirm band identity.
• The antibody is predicted to react with mouse and rat, making it suitable for rodent model studies, but cross-reactivity should be verified experimentally in your system.
• For ELISA, this antibody can be used as a capture or detection reagent; optimization of coating concentrations and detection conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and researchers with reliable access to IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-Claudin 1 antibody addresses tight junction biology, viral entry mechanisms, and skin barrier research. For rare-target sourcing or custom scale-up needs, CamelBio’s integrated supply chain bridges the gap from discovery to clinical application.