[KO Validated] CDK5 Rabbit Monoclonal Antibody - Q00535

Core Product Specifications and Parameters

Biological Background: CDK5 Function and Localization

• Cyclin-dependent kinase 5 (CDK5) is a proline-directed serine/threonine-protein kinase, also known as cell division protein kinase 5, tau protein kinase II catalytic subunit, or PSSALRE.
• CDK5 is essential for neuronal cell cycle arrest and differentiation, and plays critical roles in neuronal survival, migration, axonal and neurite growth, synaptogenesis, and synaptic plasticity.
• The kinase is activated by binding to regulatory subunits CDK5R1 (p35) and CDK5R2 (p39), primarily in postmitotic neurons, and phosphorylates a wide range of substrates including MAPT/TAU, MAP2, p53, HDAC1, and small GTPases.
• In the nucleus, CDK5 phosphorylates transcription factors such as MEF2, p53, and CLOCK, regulating gene expression, apoptosis, and circadian rhythms.
• CDK5’s subcellular localization includes the cytoplasm, nucleus, cell membrane, perikaryon, lamellipodia, growth cones, postsynaptic density, and synapses.
• Aberrant CDK5 activity, often driven by the proteolytic fragment p25, is implicated in neurodegeneration and lissencephaly, as reflected by key disease associations.
• Ubiquitously expressed, with particularly high accumulation in cortical neurons; also found in testis, skeletal muscle, colon, bone marrow, and ovary.
  Related references: PMID:17009320, PMID:19693690

Experimental Guidance and Technical Tips

• This antibody is KO validated, making it highly suitable for confirming CDK5 gene knockout in cell lines or tissues; consider using lysates from wild-type versus knockout samples as positive/negative controls.
• For Western blotting, a single band around 33 kDa is expected under reducing conditions; validate molecular weight markers and load appropriate protein amounts.
• In immunofluorescence (IF-P), CDK5 may localize to the cytoplasm, nucleus, or membrane depending on cell type and conditions; optimize fixation and permeabilization for neuronal cultures.
• For IHC-P, antigen retrieval methods and antibody dilution should be empirically determined; test on positive control tissues such as brain or skeletal muscle.
• ELISA applications may require optimized coating buffers and detection systems; perform titration experiments to establish working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted one‑stop supplier of IVD raw materials, providing diagnostic manufacturers and research laboratories with quick access to validated monoclonal antibodies like this anti‑CDK5 clone, along with bulk ancillary reagents and custom sourcing for rare targets. With deep expertise in neuroscience and oncology biomarkers, CamelBio streamlines the procurement of antibody pairs, buffers, and technical consultation to accelerate your assay development from concept to clinic.