[KO Validated] c-Myc Rabbit Monoclonal Antibody for WB, ELISA, ChIP, CUT&Tag - P01106

Core Product Specifications and Parameters

Biological Background: c-Myc Function and Localization

• c-Myc (also known as MYCC, bHLHe39, MRTL) is a proto-oncogene transcription factor belonging to the basic helix-loop-helix (bHLH) family, encoded by the MYC gene.
• It binds DNA specifically to the E-box sequence 5'-CACGTG-3' and non-specifically, activating transcription of growth-related genes including VEGFA to promote angiogenesis. Related references: PMID:24940000 PMID:25956029
• c-Myc also regulates pre-mRNA splicing by positively controlling expression of HNRNPA1, HNRNPA2, and PTBP1, which in turn modulate PKM alternative splicing. Related references: PMID:20010808
• Multiple isoforms are produced through alternative initiation and alternative splicing, contributing to functional diversity.
• It localizes to the nucleus (nucleoplasm, nucleolus), cytoplasm, and chromosomes, consistent with its roles in transcription and chromatin regulation.
• Post-translational modifications include acetylation, phosphorylation, ubiquitination (isopeptide bond), and O-glycosylation, which regulate its stability and activity.
• As a key regulator of cell proliferation and self-renewal, c-Myc is frequently overexpressed or amplified in a wide range of human cancers, making it a prominent target for diagnostic and research applications.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the N-terminal amino acids 1–100 of human c-Myc (P01106); reactivity may cover full-length and select isoforms.
• For Western blot, a band around 51 kDa is expected; include appropriate loading controls and consider cell treatment to modulate c-Myc expression.
• For ChIP and CUT&Tag, optimize antibody concentration and assay conditions (e.g., crosslinking, enzymatic digestion) with known target and non-target loci.
• Since cross-reactivity is indicated for human, verify species compatibility if studying other organisms.
• KO validation confirms specificity; nonetheless, confirm detection in relevant positive and negative control samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive one-stop platform for IVD raw materials, technical services, and consulting—guiding you from concept to clinic. For c-Myc detection in cancer and gene regulation, we supply validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing assistance. Partner with us to streamline your supply chain and advance your diagnostic or research program.