[KO Validated] ANXA1 Rabbit pAb - P04083

Core Product Specifications and Parameters

Biological Background: Annexin A1 Function and Localization

• Annexin A1 (also known as Annexin I, Lipocortin I, Calpactin II) is a calcium-dependent phospholipid-binding protein belonging to the annexin family, encoded by the ANXA1 gene (GeneID: 301).
• It functions as an effector of glucocorticoid-mediated anti-inflammatory responses, inhibiting phospholipase A2 and downregulating early inflammatory processes. Related references: PMID:8425544
• Contributes to adaptive immunity by enhancing T-cell receptor signaling and driving Th1 differentiation while suppressing Th2 differentiation. Related references: PMID:17008549
• Binds up to eight Ca²⁺ ions with high affinity and associates with phospholipid membranes in a Ca²⁺-dependent manner, facilitating membrane-related functions such as phagosome formation and cytoskeletal interactions. Related references: PMID:2532504, PMID:8557678
• Activates formyl peptide receptors (FPRs) to promote chemotaxis of granulocytes and monocytes, actin cytoskeleton rearrangement, cell polarization, and migration; also mediates resolution of inflammation and wound healing. Related references: PMID:15187149, PMID:22879591, PMID:25664854
• Negatively regulates hormone exocytosis via FPR activation and reorganization of the actin cytoskeleton. Related references: PMID:19625660
• Localizes to diverse cellular compartments including cytoplasm, nucleus, plasma membrane, endosomes, and secretory vesicles; also secreted extracellularly and present in exosomes.
• Detected in tissues such as neutrophils, T cells, placenta, and liver; elevated in extracellular vesicles from patients with inflammatory bowel disease.

Experimental Guidance and Technical Tips

• The immunogen spans the full-length human ANXA1 protein (amino acids 1-346), implying broad epitope recognition. Tested reactivity with human, mouse, and rat indicates conserved epitopes.
• In Western blot (WB), anticipate a specific band at approximately 39 kDa. Use validated positive controls (e.g., ANXA1-expressing cell lines) and optimize antibody dilution.
• For IHC-P and IF/ICC, antigen retrieval and permeabilization steps may need optimization due to ANXA1’s membrane and cytoplasmic localization.
• The antibody is KO-validated, confirming specificity, but individual experimental systems should be verified.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; include appropriate negative controls in all assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop IVD raw-material sourcing partner, providing diagnostic manufacturers and research laboratories with reliable access to validated antibodies, recombinant proteins, and ancillary reagents. For studies of inflammatory and immune responses, CamelBio offers bulk anti-ANXA1 polyclonal antibodies, matched antibody pairs, and customized sourcing of rare targets to accelerate your assay development. Our supply chain expertise spans monoclonal and polyclonal antibodies, ensuring consistent quality from research to clinical application.