[KO Validated] Anti-WSTF Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9UIG0

Core Product Specifications and Parameters

Biological Background: WSTF (BAZ1B) Function and Localization

• Atypical tyrosine-protein kinase that plays a central role in chromatin remodeling and acts as a transcription regulator. It phosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph), a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Related references: PMID:19092802 PMID:19234442
• Regulatory subunit of the ATP-dependent WICH-1 and WICH-5 ISWI chromatin remodeling complexes. These complexes form ordered nucleosome arrays, facilitate DNA access during replication, transcription, and repair, and slide mononucleosomes to the center of a DNA template. Related references: PMID:11980720 PMID:28801535
• Within the B-WICH complex, contributes to RNA polymerase III transcription. The WICH-5 ISWI complex also participates in RNA polymerase I transcription. Related references: PMID:16603771
• Mediates recruitment of the WICH-5 ISWI chromatin remodeling complex to replication foci during DNA replication. Related references: PMID:15543136
• Localizes to the nucleus, consistent with its roles in chromatin dynamics and gene regulation. It is ubiquitously expressed with high levels in heart, brain, placenta, skeletal muscle, and ovary.
• Contains bromodomain, PHD-type zinc finger, and kinase domains, and is subject to phosphorylation and acetylation. Associated with Williams-Beuren syndrome, a developmental disorder.

Experimental Guidance and Technical Tips

• The antibody was raised against the N-terminal region (amino acids 1–260) of human WSTF. Consider this immunogen design when interpreting isoform recognition or domain specificity.
• For western blotting, the predicted molecular weight is approximately 171 kDa. Use a low-percentage polyacrylamide gel and extended transfer times to ensure efficient blotting of high-molecular-weight proteins.
• This antibody is validated for WB, IHC-P, and ELISA. For IHC-P, optimize antigen retrieval and detection conditions in your specific tissue samples.
• While predicted to react with human, mouse, and rat, always validate cross-reactivity in the relevant sample system and application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to provide one-stop access to validated IVD raw materials, including high-quality antibodies like this anti-WSTF polyclonal antibody. Whether you need bulk quantities, custom development, or complementary reagents for chromatin remodeling and transcription studies, we bridge the gap from concept to clinic. Contact us for competitive sourcing solutions tailored to your workflow.