KO Validated Anti-VRK1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q99986

Core Product Specifications and Parameters

Biological Background: VRK1 Function and Localization

• Protein identity: VRK1 (Vaccinia-related kinase 1) is a widely expressed serine/threonine-protein kinase encoded by the VRK1 gene (Gene ID: 7443) in humans. It belongs to the VRK kinase family and contains conserved catalytic and regulatory domains.
• Chromatin regulation: VRK1 controls chromatin organization by phosphorylating histone H3 on Thr-4 and histone H2AX, a process critical for nuclear condensation, mitotic progression, and gene expression. Related references: PMID:31527692 PMID:37179361
• DNA damage response: In response to DNA lesions, VRK1 phosphorylates KAT5, enhancing its histone acetyltransferase activity and chromatin association, and also modifies TP53BP1 to orchestrate repair signaling. Related references: PMID:33076429 PMID:31527692
• Substrate phosphorylation and transcriptional control: VRK1 phosphorylates p53 at Thr-18, disrupting its interaction with MDM2 and stabilizing p53, while also activating ATF2 and JUN to modulate gene transcription. Related references: PMID:10951572 PMID:15105425 PMID:31527692
• Cajal body assembly: VRK1 localizes to Cajal bodies and phosphorylates coilin, a step essential for Cajal body integrity and function in RNA processing. Related references: PMID:21920476 PMID:31090908
• Golgi disassembly: During mitosis, PLK3-mediated phosphorylation of VRK1 triggers Golgi fragmentation, facilitating equal organelle partitioning into daughter cells. Related references: PMID:19103756
• Tissue expression and disease links: Highly expressed in fetal liver, testis, and thymus, VRK1 mutations are linked to neurodegenerative disorders and peripheral neuropathies, highlighting its role in neuronal maintenance.

Experimental Guidance and Technical Tips

• Immunogen design: The antibody was raised against a recombinant fragment (aa 257-396) located in the C-terminal region of human VRK1. This region exhibits moderate conservation across species; verify sequence homology if using with non-human samples.
• Western blotting: Expect a specific band at approximately 45 kDa. For increased confidence, include a knockout (KO) cell lysate as a negative control and a positive control expressing VRK1.
• IHC-P considerations: Standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) are typically suitable. Optimize antibody dilution and include tissue sections with known VRK1 expression (e.g., testis, thymus) as positive controls.
• ELISA optimization: Use recombinant VRK1 protein or peptide as coating antigen. Titrate the antibody to determine optimal working concentration and validate with appropriate blocking and detection reagents.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a strategic sourcing partner for IVD manufacturers and research institutions, offering a comprehensive range of raw materials including this polyclonal VRK1 antibody for applications in cell cycle, DNA damage, and neurobiology research. Our catalog features validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom procurement for rare targets, enabling seamless transition from early-stage development to clinical application.