KO Validated Anti-Von Hippel Lindau/VHL Rabbit Monoclonal Antibody for WB, ELISA - P40337

Core Product Specifications and Parameters

Biological Background: Von Hippel Lindau/VHL Function and Localization

• pVHL is a substrate recognition component of the VCB-Cul2 E3 ubiquitin ligase complex, mediating oxygen-dependent ubiquitination of target proteins for proteasomal degradation.
• It specifically ubiquitinates hypoxia-inducible factor alpha subunits (HIF1A, HIF2A) under normoxia, leading to HIF degradation and repression of hypoxia-responsive genes. Related references: PMID:10944113, PMID:17981124
• VHL also modulates mTORC1 signaling by promoting ubiquitination and degradation of RPTOR, and regulates adrenergic receptor ADRB2 degradation in an oxygen-dependent manner. Related references: PMID:19584355, PMID:34290272
• Subcellularly, VHL localizes to the cytoplasm, cell membrane, endoplasmic reticulum, and nucleus, consistent with its roles in protein degradation and transcriptional control.
• The protein is expressed in the adult and fetal brain and kidney, with disease-associated mutations linked to von Hippel-Lindau syndrome, congenital erythrocytosis, and cancer.
• Keywords: Tumor suppressor, alternative initiation, alternative splicing, disease variant.

Experimental Guidance and Technical Tips

• For Western blot, consider using a reducing SDS-PAGE and transfer to PVDF or nitrocellulose membrane. The predicted molecular weight is approximately 24 kDa, so use a 10–12% gel for optimal resolution.
• Validate antibody specificity using VHL knockout cell lines or siRNA-mediated knockdown samples in human, mouse, or rat cells, given the cross-reactivity.
• For ELISA, this antibody can be used as a capture or detection reagent; pairing with a monoclonal antibody of a different clonality may enhance sensitivity in sandwich ELISA formats.
• Optimize antibody dilution in the relevant assay system; typical starting dilutions for WB are 1:500–1:2000, but empirical titration is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, covering every stage from concept to clinic. For VHL-related research and assay development, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing. Our integrated technical services and consulting help accelerate your VHL-targeted IVD projects from biomarker discovery to final product deployment.