[KO Validated] Anti-VAT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q99536

Core Product Specifications and Parameters

Biological Background: VAT1 Function and Localization

• VAT1 is a multifunctional protein with both enzymatic and transport activities. Related references: PMID:17105775 PMID:26394711 PMID:32005660
• It acts as a probable NADPH-dependent quinone oxidoreductase, though its physiological substrates and specific function remain unclear. Related references: PMID:33483563
• Alternatively, VAT1 has been proposed to mediate the transfer of negatively charged phospholipids, including phosphatidic acid, phosphatidylserine, and phosphatidylglycerol, between the endoplasmic reticulum and mitochondrial membranes. Related references: PMID:26394711 PMID:32005660
• Through its interaction with mitofusins, VAT1 may regulate mitochondrial fusion. Related references: PMID:17105775
• The protein localizes to the cytoplasm, cytosol, and mitochondrial outer membrane.
• VAT1 is expressed in the brain and in glioblastoma cells.
• Post-translational modifications include acetylation and phosphorylation; the gene also undergoes alternative splicing.
• Keywords associated with VAT1 include lipid transport, oxidoreductase, and reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to an internal region (aa 200–300) of human VAT1; consider verifying sequence identity in your samples to ensure specificity.
• For western blotting, a band detected around 42 kDa is anticipated; confirm specificity using positive controls and consider performing a knockout validation to confirm the band.
• In immunofluorescence, VAT1 may localize to both cytoplasmic and mitochondrial compartments; appropriate blocking and permeabilization steps are recommended to visualize these structures.
• For ELISA, titrate antibody concentration against the immunogen peptide or recombinant protein to determine optimal working conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research teams with comprehensive access to high-quality raw materials like this anti-VAT1 polyclonal antibody, targeting a key player in mitochondrial biology and lipid transport. Our platform streamlines sourcing of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and even rare-target raw materials, supporting workflows from concept to clinic. Explore VAT1-related tools to advance your studies in cellular metabolism and neurological research.