KO Validated Anti-TXNDC9 Polyclonal Antibody for WB and ELISA - O14530

Core Product Specifications and Parameters

Biological Background: TXNDC9 Function and Localization

• TXNDC9, also known as APACD (ATP-binding protein associated with cell differentiation) and Protein 1-4, is encoded by the TXNDC9 gene.
• It functions as a negative regulator of the chaperonin TCP1 complex (CCT/TRiC) ATPase activity, impairing protein folding of substrates like actin and tubulin.
• The protein localizes to the cytoplasm and nucleus, with specific accumulation at the centrosome and midbody, linking it to cytoskeletal organization and cell division.
• Post-translational modifications include phosphorylation, and alternative splicing generates multiple transcript variants.

Experimental Guidance and Technical Tips

• For Western blot, use whole cell lysates from human, mouse, or rat cells; expect a band near 27 kDa. Include KO and wild-type controls to confirm specificity.
• For ELISA, the antibody can be used as a capture or detection reagent; optimize coating with recombinant TXNDC9 or its immunogen and perform cross-titration.
• Because the immunogen targets the N-terminal region (aa 1-110), the antibody likely recognizes most isoforms; however, validate in your specific sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with a single point of access to high-quality IVD raw materials, including validated polyclonal antibodies like this anti-TXNDC9 product, which supports studies on protein folding and cytoskeleton dynamics. Our portfolio covers antibody pairs, bulk ancillary reagents, and custom sourcing for even the most challenging targets, streamlining your development from concept to clinic.