[KO Validated] Anti-TTC11/FIS1 Rabbit Polyclonal Antibody for WB and ELISA - Q9Y3D6

Core Product Specifications and Parameters

Biological Background: FIS1 Function and Localization

• Mitochondrial fission 1 protein (FIS1), also known as TTC11 or hFis1, is a key mediator of mitochondrial and peroxisomal fission.
• It is involved in the fragmentation of the mitochondrial network and its perinuclear clustering, playing a role in mitochondrial dynamics. Related references: PMID:12783892 PMID:12861026 PMID:14996942
• FIS1 has a minor role in recruiting dynamin-related protein 1 (DNM1L) to the mitochondrial surface but may not be essential for fission complex assembly. Related references: PMID:12861026 PMID:16118244 PMID:23283981 PMID:23530241 PMID:24196833
• It also mediates peroxisomal fission, extending its role beyond mitochondrial dynamics. Related references: PMID:16107562
• FIS1 can induce cytochrome c release from mitochondria, ultimately leading to apoptosis. Related references: PMID:12783892
• Subcellular localization: mitochondrial outer membrane and peroxisome membrane.
• The protein contains a tetratricopeptide repeat (TPR) domain and a single transmembrane helix.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, indicating regulatory complexity.

Experimental Guidance and Technical Tips

• The immunogen comprises the N-terminal region (residues 1–122) of human FIS1, which includes the cytoplasmic TPR domain; this design may favor recognition of native FIS1 without interference from the transmembrane region.
• For Western blotting, consider enriching mitochondrial fractions to enhance signal specificity.
• Validate antibody reactivity in lysates from human, mouse, and rat cells, and include appropriate positive and negative controls.
• For ELISA, recombinant FIS1 or the immunogen peptide can be used as coating antigen; optimise coating conditions and antibody dilution.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutes seamless access to this anti-FIS1 polyclonal antibody and a comprehensive portfolio of IVD raw materials. Whether you require validated antibody pairs, optimised monoclonal/polyclonal antibodies, bulk ancillary reagents, or rare-target sourcing, our one-stop supply chain supports your mitochondrial dynamics and apoptosis research from concept to clinic.