[KO Validated] Anti-TTC11/FIS1 Rabbit Monoclonal Antibody for WB - Q9Y3D6

Core Product Specifications and Parameters

Biological Background: TTC11/FIS1 Function and Localization

• TTC11/FIS1, also known as Mitochondrial fission 1 protein (hFis1) or Tetratricopeptide repeat protein 11, is encoded by the FIS1 (TTC11) gene in humans and contains TPR repeat domains.
• It localizes to the mitochondrial outer membrane and peroxisome membrane, where it plays a role in organelle fission.
• FIS1 is involved in the fragmentation of the mitochondrial network and its perinuclear clustering, promoting mitochondrial fission. Related references: PMID:12783892, PMID:12861026, PMID:14996942
• It recruits fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface, though it may not be essential for fission complex assembly. Related references: PMID:12861026, PMID:16118244, PMID:23283981, PMID:23530241, PMID:24196833
• FIS1 also mediates peroxisomal fission, indicating a dual role in organelle dynamics. Related references: PMID:16107562
• Depending on cellular context, FIS1 may direct fission products toward mitochondrial homeostasis, mitophagy, or apoptosis. Related references: PMID:24196833
• Overexpression of FIS1 can induce cytochrome c release from mitochondria and trigger apoptosis. Related references: PMID:12783892
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, as identified by proteomics.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal amino acids 1–122, which contains the TPR motifs and is predicted to be cytoplasmic and accessible. Consider this region when designing epitope-tagging or competition assays.
• For Western blot, expect a band near 17 kDa. Optimize loading controls and blocking conditions for your target tissue or cell line.
• For immunohistochemistry (IHC), antigen retrieval may be needed; titrate the antibody to determine optimal working dilution.
• In immunofluorescence (IF/ICC), permeabilization is required to detect the mitochondrial and peroxisomal membrane protein. Co-stain with organelle markers to confirm localization.
• This rabbit monoclonal antibody is suitable for immunoprecipitation (IP); validate the elution buffer and wash conditions in your system.
• For ELISA, the antibody can be used as a capture or detection reagent; check pairing compatibility if used with other antibodies.
• Cross-reactivity with mouse and rat is predicted due to sequence homology; verify signal specificity in each species using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target procurement. This TTC11/FIS1 monoclonal antibody supports mitochondrial and peroxisomal fission research, as well as apoptosis studies, helping you streamline assay development and production workflow.