[KO Validated] Anti-TRIM21/SS-A Rabbit Monoclonal Antibody for WB and ELISA - P19474

Core Product Specifications and Parameters

Biological Background: TRIM21 Function and Localization

• TRIM21 (also known as Ro52, SSA1, RNF81) is a 54 kDa E3 ubiquitin-protein ligase and a member of the tripartite motif (TRIM) family, characterized by RING finger, B-box, and coiled-coil domains.
• It functions as an E3 ubiquitin ligase dependent on E2 enzymes UBE2D1, UBE2D2, UBE2E1, and UBE2E2, forming a complex with UBE2D2 that mediates self-ubiquitination and ubiquitination of substrates such as USP4 and IKBKB. Related references: PMID:16297862, PMID:16316627, PMID:16472766, PMID:16880511, PMID:19675099
• TRIM21 is a component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes, such as SCF(SKP2)-like complexes, which ubiquitinate CDKN1B phosphorylated at Thr-187, promoting its proteasomal degradation and regulating cell cycle progression. Related references: PMID:16880511
• It negatively regulates innate immune signaling by targeting IRF3 for polyubiquitin-mediated degradation, dampening IFN-beta production after pathogen recognition, and also monoubiquitinates IKBKB to inhibit Tax-induced NF-kappa-B signaling. Related references: PMID:18641315, PMID:19675099
• TRIM21 mediates ubiquitin-dependent proteasomal degradation of IgG1 heavy chain via the ER-associated degradation (ERAD) pathway and enhances the decapping activity of DCP2. Related references: PMID:18022694, PMID:18361920
• It plays a key role in autophagy regulation by serving as a platform for the assembly of ULK1, Beclin 1, and ATG8 family members, and it targets FIP200/RB1CC1 for ubiquitination to modulate autophagy. Related references: PMID:26347139, PMID:36359729
• TRIM21 localizes to the cytoplasm, nucleus, P-bodies, stress granules, and autophagosomes, and it exists as a ribonucleoprotein particle that binds small HY RNAs, with tissue-specific isoforms expressed in fetal and adult heart and fetal lung. Related references: PMID:1985094, PMID:8666824
• During viral infection, it promotes cell pyroptosis via 'Lys-6'-linked ubiquitination of ISG12a/IFI27, and it positively regulates innate immunity by 'Lys-27'-linked ubiquitination of MAVS. Moreover, it mediates 'Lys-63'-linked polyubiquitination of G3BP1 in response to heat shock, leading to stress granule disassembly. Related references: PMID:36426955, PMID:29743353, PMID:36692217

Experimental Guidance and Technical Tips

• For Western blot, a band around 54 kDa is expected; validate band specificity using knockout or knockdown cell lysates.
• Optimize primary antibody dilution in WB and ELISA according to your specific experimental conditions, as the optimal working concentration may vary.
• Since the immunogen corresponds to amino acids 86-334, the antibody likely recognizes a region common to both isoforms 1 and 2, which are expressed in heart and lung tissues.
• When using rat samples, confirm cross-reactivity by testing a panel of tissues, as reactivity has been observed but may need verification for your target tissue.

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