[KO Validated] Anti-TMED10 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P49755

Core Product Specifications and Parameters

Biological Background: TMED10 Function and Localization

• TMED10 (also known as TMP21, p23, p24δ1) is a member of the p24 family of type I transmembrane cargo receptors, playing essential roles in protein vesicular trafficking between the ER and Golgi.
• TMED10 cycles between the endoplasmic reticulum (ER), ER-Golgi intermediate compartment (ERGIC), and Golgi apparatus, mediating cargo transport through both COPI and COPII-coated vesicles.
  Related references: PMID:10052452, PMID:10852829, PMID:12237308
• In COPII anterograde transport, TMED10 collaborates with TMED2 as a cargo receptor for GPI-anchored proteins, recognizing remodeled GPI anchors after processing by PGAP1 and PGAP5; this function involves SEC24C/D and ER lipid rafts.
  Related references: PMID:20427317, PMID:27569046
• In COPI retrograde transport, TMED10 participates in vesicle biogenesis and coat recruitment; on Golgi membranes, it acts as a receptor for ARF1-GDP and modulates ARFGAP2’s GTPase-activating activity.
  Related references: PMID:11726511, PMID:19296914
• TMED10 associates with the presenilin-dependent gamma-secretase complex and influences amyloid beta A4 protein processing, regulating Aβ40 production and affecting APP trafficking and soluble APP-beta release.
  Related references: PMID:16641999, PMID:17288597
• Beyond its cargo receptor role, TMED10 can oligomerize to form a protein channel, facilitating the post-translational entry of leaderless cytoplasmic cargos (e.g., IL-1β, HSPB5, galectins) into ERGIC for unconventional secretion; this process is chaperone-assisted.
  Related references: PMID:32272059
• Subcellularly, TMED10 resides primarily in early secretory pathway membranes: ER, ERGIC, cis- and trans-Golgi, but is also detected at the cell membrane, in cytoplasmic vesicles, and melanosomes.

Experimental Guidance and Technical Tips

• The immunogen spans the N-terminal lumenal/extracellular domain (amino acids 35–190) of human TMED10; as a polyclonal antibody, it may recognize multiple epitopes and possibly detect isoforms or processed forms.
• In Western blot (WB), expect a specific band at approximately 25 kDa in lysates from human, mouse, and rat cell lines or tissues; consider potential glycosylation or other modifications that may shift the apparent molecular weight.
• For immunohistochemistry (IHC-P) and immunocytochemistry (IF/ICC), TMED10 typically shows a perinuclear ER/Golgi-like staining pattern; optimize fixation, permeabilization, and blocking steps with appropriate positive control tissues or cells.
• When using ELISA, titrate the antibody and validate coating and detection conditions; the polyclonal nature may provide robust signal but requires careful lot-specific standardization.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical quantitative applications; consider using a control antigen or reference standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a premier one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. For targets like TMED10, involved in vesicular trafficking and secretory pathways, we offer a comprehensive portfolio of validated antibodies (both polyclonal and monoclonal) and bulk ancillary reagents. Our expert team ensures seamless procurement of rare-target raw materials and provides technical support to accelerate your assay development.