[KO Validated] Anti-TFAP4 Polyclonal Antibody for WB, IF/ICC, ELISA - Q01664

Core Product Specifications and Parameters

Biological Background: TFAP4 Function and Localization

• TFAP4 (Transcription factor AP-4), also designated AP-4 or bHLHc41, belongs to the class C basic helix-loop-helix (bHLH) transcription factor family.
• It functions as a transcriptional activator by binding to the palindromic DNA sequence 5'-CAGCTG-3', regulating both viral and cellular gene expression.
• The protein is predominantly localized to the nucleus, consistent with its function in transcriptional control.
• TFAP4 contains a conserved bHLH domain that mediates DNA binding and dimerization, characteristic of the bHLHc subfamily.
• Post-translational modifications include phosphorylation and ubiquitination (Ubl conjugation), which may modulate its activity and stability; isopeptide bond formation is noted in UniProt.
• The calculated molecular weight is approximately 38.7 kDa (UniProt), and it migrates at ~39 kDa on SDS-PAGE.

Experimental Guidance and Technical Tips

• For Western blot, an expected band around 39 kDa should be observed; optimize antibody dilution (e.g., 1:500–1:2000) and consider using a suitable loading control.
• In immunofluorescence/immunocytochemistry, nuclear staining is anticipated; perform permeabilization and blocking steps appropriate for the cell type.
• For ELISA, the peptide immunogen or recombinant TFAP4 protein may be used as coating antigen; test serial dilutions to determine optimal working concentration.
• As a rabbit polyclonal antibody, it may recognize multiple epitopes; validate specificity in knockout or knockdown systems if available.
• Cross-reactivity has been verified for human and rat; for other species, homology of the immunogen sequence should be checked before use.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in providing diagnostic manufacturers and research laboratories with high-quality IVD raw materials and end-to-end support. The TFAP4 polyclonal antibody is part of our extensive catalog for transcription factor research, and we offer validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets. From antibody selection to technical consultation, CamelBio accelerates your assay development and product commercialization.