[KO Validated] Anti-SUZ12 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - Q15022

Core Product Specifications and Parameters

Biological Background: SUZ12 Function and Localization

• SUZ12 is a core component of the Polycomb repressive complex 2 (PRC2), which catalyzes methylation of histone H3 at lysine 9 (H3K9me) and lysine 27 (H3K27me), leading to transcriptional gene silencing. Related references: PMID:15225548, PMID:15231737, PMID:15385962
• The PRC2 complex may recruit DNA methyltransferases, linking histone methylation to DNA methylation and reinforcing epigenetic repression. Related references: PMID:12351676, PMID:12435631, PMID:15099518
• Known target genes repressed by SUZ12-containing PRC2 include HOXC8, HOXA9, MYT1, and CDKN2A. Related references: PMID:15231737, PMID:16618801, PMID:17200670
• SUZ12 localizes exclusively to the nucleus, consistent with its role in chromatin regulation.
• It is overexpressed in breast and colon cancer, suggesting a role in oncogenesis.
• Post‑translational modifications include phosphorylation, isopeptide bond formation, and ubiquitination (as inferred from UniProt keywords: Phosphoprotein, Isopeptide bond, Ubl conjugation).
• Additionally, SUZ12 contains a zinc finger domain and belongs to the Polycomb group family of transcriptional repressors.

Experimental Guidance and Technical Tips

• For Western blotting, a band at approximately 83 kDa is expected; validate in your sample system.
• For immunofluorescence, expect nuclear localization; optimization of fixation and permeabilization may be required.
• This antibody is described as KO‑validated; specificity can be confirmed using knockout cell lines.
• In immunoprecipitation, it may enrich the entire PRC2 complex; suitable for analyzing protein‑protein interactions.
• When adapting to ELISA, consider the recombinant immunogen fragment (aa 470‑739) as a positive control.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio serves as a one‑stop sourcing partner for IVD raw materials, offering validated polyclonal antibodies like this anti‑SUZ12 antibody for epigenetic and cancer research. Beyond individual reagents, we provide optimized antibody pairs, bulk ancillary reagents, and specialized raw‑material sourcing to streamline your assay development from concept to clinic.