[KO Validated] Anti-STRAP Rabbit Polyclonal Antibody for WB and ELISA - Q9Y3F4

Core Product Specifications and Parameters

Biological Background: STRAP Function and Localization

• STRAP (serine-threonine kinase receptor-associated protein) is also known as MAWD (MAP activator with WD repeats), UNRIP (UNR-interacting protein), and PT-WD (WD-40 repeat protein PT-WD).
• It functions in the SMN (survival motor neuron) complex, facilitating the assembly of small nuclear ribonucleoproteins (snRNPs) essential for pre-mRNA splicing.
• The SMN complex accepts 5Sm proteins from the chaperone CLNS1A to form an intermediate, then binds snRNA to trigger eviction and complete core snRNP assembly.
• STRAP negatively regulates TGF-beta signaling, although the exact mechanism is unclear.
• It positively regulates PDPK1 kinase activity by enhancing autophosphorylation and reducing the association of PDPK1 with 14-3-3 protein.
• The protein localizes to both the cytoplasm and nucleus, consistent with its roles in signaling and splicing.
• As a phosphoprotein with multiple WD repeats, post-translational modifications and alternative splicing may generate additional isoforms.
• The predicted molecular weight is approximately 38.4 kDa, matching the observed 38 kDa band on SDS-PAGE.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a recombinant protein spanning the N-terminal 350 amino acids of human STRAP, likely recognizing full-length endogenous STRAP.
• For Western blot (WB), use standard protocols with a recommended starting dilution of interest; a strong band at ~38 kDa is expected. Validate in your specific sample system.
• For ELISA, consider direct or indirect formats; this antibody may serve as a capture or detection reagent after proper optimization.
• It cross-reacts with mouse and rat STRAP; however, we recommend sequence alignment and experimental confirmation for other species.
• As a polyclonal antibody, lot-to-lot consistency is possible but should be monitored; include positive and negative controls in each assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for diagnostic and research communities, offering one-stop access to premium IVD raw materials. This STRAP antibody is ideally suited for studies on spliceosome assembly, TGF-beta signaling, and protein kinase regulation. Beyond antibodies, CamelBio supplies validated antibody pairs, bulk ancillary reagents, and specialized sourcing for rare targets, supporting your projects from concept to clinic.