[KO Validated] Anti-STAT5B Rabbit Monoclonal Antibody for WB, IP, ELISA - P51692

Core Product Specifications and Parameters

Biological Background: STAT5B Function and Localization

• STAT5B is a signal transducer and transcription activator that mediates cellular responses to cytokines, hormones, and growth factors.
  Related references: PMID:29844444, PMID:9188471

• It binds to the GAS element and activates prolactin (PRL)-induced transcription.

• STAT5B positively regulates hematopoietic and erythroid differentiation.

• Involved in oncostatin-M-mediated signaling through the type II OSM receptor complex (OSMR/IL6ST).
  Related references: PMID:9188471

• Upon activation, STAT5B translocates from the cytoplasm to the nucleus to regulate gene expression.

• The protein features an SH2 domain and is regulated by phosphorylation (phosphoprotein).

• Mutations in STAT5B are linked to growth hormone insensitivity with immunodeficiency (dwarfism).

Experimental Guidance and Technical Tips

• The immunogen (aa 600-787) encompasses the C-terminal SH2 domain and key phosphorylation sites, making the antibody potentially suitable for detecting both total and phosphorylated STAT5B; confirm specificity via phosphatase-treated controls or relevant inhibitors.

• For Western blot, expect a band at ~90 kDa; validate in lysates from hematopoietic cell lines or tissues with known STAT5B expression.

• For immunoprecipitation, consider cross-linking the antibody to protein A/G beads to minimize heavy/light chain interference.

• For ELISA, pairing with a capture antibody against a distinct epitope may improve sensitivity; optimize coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined one-stop channel for IVD raw material sourcing, from antibody discovery to bulk supply. This anti-STAT5B antibody supports the development of assays targeting cytokine signaling pathways and hematopoietic disorders. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized sourcing solutions to accelerate your project from concept to clinic.