KO Validated Anti-Src Monoclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P12931

Core Product Specifications and Parameters

Biological Background: Src Function and Localization

• Proto-oncogene Src (c-Src) is a non-receptor tyrosine kinase that integrates signals from diverse receptors (immune, integrin, RTK, GPCR) to regulate gene transcription, cell cycle, adhesion, and migration.
• Src phosphorylates focal adhesion proteins (e.g., PTK2/FAK1, paxillin) and cortactin to orchestrate cytoskeletal dynamics. Related references: PMID:21411625
• At cell junctions, Src phosphorylates beta-catenin, connexin-43, and desmosomal PKP3, influencing cell-cell communication and adhesion. Related references: PMID:25501895
• Src is essential for osteoclast-mediated bone resorption via complex formation with PTK2B/PYK2 and CBL phosphorylation pathway. Related references: PMID:14585963, PMID:8755529
• The kinase shuttles between plasma membrane, focal adhesions, cytoplasm, nucleus, and mitochondrion; myristoylation anchors it to membranes.
• Alternative splicing produces isoforms with higher basal activity (e.g., neuron-specific isoforms 2/3) that regulate neurite elongation and show distinct substrate specificity.
• Src activity is tightly regulated by autophosphorylation (Tyr-419) and negative-regulatory C-terminal tail phosphorylation.

Experimental Guidance and Technical Tips

• The immunogen sequence (aa 1-100) resides in the N-terminal unique domain, which is less conserved among Src family kinases, potentially contributing to Src specificity.
• For Western blot, expect a band at ~60 kDa; use appropriate controls (e.g., Src knockout lysate) to confirm specificity.
• The antibody is suitable for immunoprecipitation; consider using mild lysis buffers to preserve protein interactions if studying signaling complexes.
• For immunofluorescence and IHC, endogenous Src may be detected at the plasma membrane, focal adhesions, and perinuclear region; optimize fixation and permeabilization conditions.

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