[KO Validated] Anti-SOD2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P04179

Core Product Specifications and Parameters

Biological Background: SOD2 Function and Localization

• Superoxide dismutase [Mn], mitochondrial (SOD2) is an oxidoreductase that catalyzes the dismutation of superoxide anion radicals into oxygen and hydrogen peroxide.
• It is a manganese-dependent enzyme, with the metal ion essential for its catalytic function.
• The protein is localized exclusively to the mitochondrial matrix, as annotated by the transit peptide and subcellular location evidence.
• SOD2 is subject to acetylation and nitration, post-translational modifications that can regulate its enzymatic activity.
• The human SOD2 gene undergoes alternative splicing, yielding multiple transcript variants.
• Direct protein sequencing and proteomics identification confirm the endogenous expression and sequence of the protein across tissues.
• The mature protein has a calculated molecular weight of approximately 24.8 kDa, consistent with the observed band at 25 kDa.

Experimental Guidance and Technical Tips

• For Western blotting, load positive control lysates from human, mouse, or rat cells; expect a band at approximately 25 kDa. Use KO-validated samples to confirm specificity.
• In immunohistochemical paraffin sections (IHC-P), antigen retrieval with citrate buffer (pH 6.0) is often effective for mitochondrial antigens; optimize detection with a sensitive polymer-based method.
• For immunofluorescence (IF/ICC), permeabilize cells and include a mitochondrial marker to confirm subcellular co-localization.
• The antibody is suitable for sandwich ELISA as a capture or detection reagent; validate pairing with a matched antibody for the target.
• Since the immunogen corresponds to the mature protein (a.a. 25-222), the antibody is expected to detect endogenous full-length SOD2 but not the precursor with transit peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For researchers studying oxidative stress and mitochondrial function, CamelBio offers validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents to accelerate assay development. Our global procurement network can also source rare target raw materials not listed in the standard catalog.