KO Validated Anti-Smad1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q15797

Core Product Specifications and Parameters

Biological Background: Smad1 Function and Localization

• Protein identity: SMAD family member 1 (Smad1), also known as BSP-1, JV4-1, MADH1, MADR1. Functions as a transcriptional modulator in embryonic development, cell differentiation, and tissue homeostasis. Related references: PMID:9335504

• Upon BMP ligand binding, Smad1 is phosphorylated by activated type I BMP receptors, then associates with SMAD4 to form a heteromeric complex that translocates to the nucleus and acts as a transcription factor. Related references: PMID:33667543

• The nuclear Smad1/SMAD4 complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate gene expression. Related references: PMID:33667543

• Smad1, in complex with OAZ1 and PSMB4, mediates the degradation of the CREBBP/EP300 repressor SNIP1, linking BMP signaling to transcriptional coactivator regulation.

• Smad1 positively regulates BMP4-induced expression of the odontogenic development regulator MSX1 following IPO7-mediated nuclear import.

• Subcellular localization is both cytoplasmic and nuclear, consistent with its role in signal transduction from the cell membrane to the nucleus.

• Tissue expression is ubiquitous, with highest levels observed in heart and skeletal muscle.

• Post-translational modifications include phosphorylation, acetylation, and ubiquitination; it contains a zinc-binding domain and functions as a DNA-binding transcription factor.

Experimental Guidance and Technical Tips

• Based on the immunogen design (amino acids 20-240 of human Smad1), the antibody may recognize both unphosphorylated and phosphorylated forms; consider assessing phospho-specificity with appropriate controls.
• For Western blot, an expected band at approximately 52 kDa should be observed; use tissue or cell lysates with high Smad1 expression (e.g., heart or skeletal muscle) as positive controls.
• KO validation suggests suitability for confirming target specificity in knockout cell lines or tissues; include a wild-type control where possible.
• For IHC-P and IF/ICC, optimize antigen retrieval and antibody dilution using known positive tissue samples; cross-reactivity with mouse and rat allows use in rodent models.
• As a polyclonal antibody, lot-to-lot consistency may vary; validate each new batch in the intended application and always include appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For BMP signaling and transcription regulation research, we offer validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-Smad1 antibody, and bulk ancillary reagents to support your assay development. Our team can also assist with rare target raw-material sourcing, ensuring you have a reliable supply chain from target identification to final product.