[KO Validated] Anti-SLC25A4/ANT1 Rabbit Polyclonal Antibody - P12235

Core Product Specifications and Parameters

Biological Background: SLC25A4/ANT1 Function and Localization

• Functions as an ADP:ATP antiporter, importing ADP into the mitochondrial matrix for ATP synthesis while exporting ATP to fuel cellular processes. Related references: PMID:21586654, PMID:27693233, PMID:23173940

• Operates via an alternating access mechanism, transitioning between cytoplasmic-open (c-state) and matrix-open (m-state) conformations; substrate exchange occurs consecutively with ordered binding and release. Related references: PMID:37278158 (By similarity)

• Exhibits proton transporter activity that mediates mitochondrial uncoupling, reducing ATP production efficiency and contributing to thermogenesis; this activity requires free fatty acids as cofactors. Related references: PMID:31883789 (By similarity)

• Plays a key role in opening the mitochondrial permeability transition pore (mPTP), a non-specific pore linked to cell death; whether ANT1 is a direct pore component or a regulator remains under investigation. Related references: PMID:31883789 (By similarity)

• Regulates mitophagy independently of its antiporter activity through interaction with TIMM44, which inhibits the presequence translocase TIMM23 and stabilizes PINK1 (By similarity).

• Localizes to the mitochondrial inner membrane as a multi-pass transmembrane protein; expressed at the protein level in erythrocytes.

• Subject to multiple post-translational modifications including acetylation, methylation, phosphorylation, and S-nitrosylation; disease-causing variants are associated with cardiomyopathy and progressive external ophthalmoplegia.

Experimental Guidance and Technical Tips

• The immunogen spans residues 101–200 within the ANT1 sequence; confirm epitope accessibility in your assay setup and consider that endogenous protein conformation may affect binding.
• For Western blotting, a specific band around 33 kDa is expected; standard reducing SDS-PAGE conditions are typically suitable, but optimization of sample preparation (e.g., boiling time) may be needed.
• In immunofluorescence/ICC, expect a mitochondrial staining pattern; co-staining with mitochondrial markers (e.g., MitoTracker or Tom20) is recommended to validate localization.
• This antibody is KO-validated, providing assurance of specificity; include appropriate knockout or knockdown controls in your experiments to confirm target recognition.
• The antibody reacts with human, mouse, and rat; sequence homology suggests likely reactivity with other mammals, but verification is advised for other species.
• For ELISA, consider pairing with a capture/detection antibody that binds a non-overlapping epitope; optimization of coating and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive partner for IVD raw materials, offering validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing to support mitochondrial research and energy metabolism studies. This anti-ANT1 antibody provides a reliable tool for investigating adenine nucleotide translocation and mitochondrial function, and we can assist with additional reagents or custom development to accelerate your diagnostic and research projects. Rely on CamelBio for efficient, end-to-end supply solutions from concept to clinic.