KO Validated Anti-SLC25A22 Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H936

Core Product Specifications and Parameters

Biological Background: SLC25A22 Function and Localization

• SLC25A22 (mitochondrial glutamate carrier 1, GC1) is a member of the solute carrier family 25, also known as glutamate/H(+) symporter 1, and is encoded by the gene SLC25A22 (aliases: GC1, EIEE3, NET44).
• It functions as a mitochondrial glutamate/H(+) symporter, catalyzing the electroneutral cotransport of L-glutamate and a proton across the inner mitochondrial membrane from the cytosol into the matrix.
  Related references:
  PMID:11897791
• In pancreatic beta cells, GC1 contributes to the regulation of glucose-stimulated insulin secretion by supplying glutamate to the mitochondrial matrix, linking metabolic signals to hormone release.
• The SLC25A22 protein is localized to the inner mitochondrial membrane, consistent with its role in mitochondrial transport processes.
• Tissue expression studies show high levels of SLC25A22 mRNA in the brain, liver, and pancreas, tissues with high metabolic and neurotransmitter demand.
• Mutations in SLC25A22 cause early infantile epileptic encephalopathy (EIEE3), highlighting its critical role in neuronal function.
• The protein contains characteristic mitochondrial carrier family repeat domains, each forming two transmembrane helices, a structural hallmark shared by all SLC25 members.
• Keyword annotations from UniProt include: Disease variant, Epilepsy, Membrane, Mitochondrion inner membrane, Symport, and Transport.

Experimental Guidance and Technical Tips

• The provided immunogen corresponds to amino acids 128–188 of human SLC25A22, a region that may lie in a loop between transmembrane domains; this sequence is highly conserved among human, mouse, and rat, supporting cross-species reactivity.
• For western blot applications, use mitochondrial-enriched lysates from brain, liver, or pancreatic cells to detect the endogenous ~34 kDa band. Include a KO‑validated negative control to confirm band specificity.
• In immunofluorescence/immunocolocalization (IF/ICC), expect a mitochondrial staining pattern; co‑stain with a mitochondrial marker (e.g., TOMM20) to verify localization.
• For ELISA, the recombinant immunogen can serve as a standard or coating antigen; titrate the antibody in direct or sandwich ELISA formats.
• Store the antibody in aliquots at –20 °C; avoid repeated freeze‑thaw cycles, especially if shipped on ice packs.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and clinical institutes streamlined access to validated IVD raw materials, including mitochondrial carrier antibodies such as anti‑SLC25A22. We supply bulk polyclonal and monoclonal antibodies, matched antibody pairs, and ancillary reagents tailored for WB, IF/ICC, and ELISA workflows. Whether scaling up production or sourcing rare targets, CamelBio bridges your concept to the clinic with technical expertise and a reliable global supply chain.