[KO Validated] Anti-SIRT1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96EB6

Core Product Specifications and Parameters

Biological Background: SIRT1 Function and Localization

• NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics, coordinating cellular processes such as cell cycle, DNA damage response, metabolism, apoptosis, and autophagy. Related references: PMID:11672523
  PMID:12006491
  PMID:14976264

• Modulates chromatin function through deacetylation of histones H3, H4, H2A, and H1-4, and promotes alterations in histone methylation leading to transcriptional repression. Related references: PMID:15469825

• Deacetylates a broad range of transcription factors including p53/TP53, FOXO3, RELA/NF-κB p65, and MYC, thereby regulating target gene expression both positively and negatively in processes such as apoptosis, stress resistance, and metabolism. Related references: PMID:11672523
  PMID:12006491
  PMID:14976264
  PMID:14980222
  PMID:15152190
  PMID:19364925

• Serves as a sensor of the cytosolic NAD+/NADH ratio, linking nutrient status to gene expression; essential for skeletal muscle cell differentiation and mediates the response to caloric restriction. Related references: PMID:15205477
  PMID:23142079

• Plays a critical role in DNA damage repair by deacetylating repair proteins such as XRCC6/Ku70 and WRN, and facilitating recruitment of factors to damaged DNA. Related references: PMID:15205477
  PMID:16998810
  PMID:17334224
  PMID:18203716

• Localizes to the nucleus, PML bodies, cytoplasm, and mitochondria; widely expressed across tissues. (No PubMed references for localization)

• During HIV-1 infection, interacts with and deacetylates the viral Tat protein, which inhibits SIRT1 activity toward RELA/NF-κB p65, contributing to T-cell hyperactivation. (No PubMed references for this specific interaction)

• Exists in multiple isoforms: isoform 1 is catalytically active, isoform 2 has lower activity and regulates p53 expression in an autoregulatory loop, and 75SirT1 is catalytically inactive and may protect chondrocytes from apoptosis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 448-747 in the C-terminal region of human SIRT1, a segment well-conserved across human, mouse, and rat, consistent with the observed cross-reactivity.
• In Western blotting (WB), the predicted molecular weight is approximately 82 kDa; due to alternative splicing, post-translational modifications, or known isoforms, additional bands may be detected.
• For immunofluorescence/immunocytochemistry (IF/ICC), verify the staining pattern in your cell type of interest, as SIRT1 can shuttle between nucleus, cytoplasm, and mitochondria depending on cellular conditions.
• For ELISA, consider pairing with a capture antibody or using as a detection antibody; validate in the appropriate sample matrix, as performance may vary with assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive access to IVD raw materials, including validated antibody pairs, high-quality monoclonal and polyclonal antibodies, and bulk ancillary reagents for metabolism, cancer, and aging research. This anti-SIRT1 polyclonal antibody addresses key targets in molecular biology and disease mechanism studies. Additionally, CamelBio offers rare target raw-material sourcing and technical consulting to streamline your workflow from discovery to clinical application.