[KO Validated] Anti-SETD7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q8WTS6

Core Product Specifications and Parameters

Biological Background: SETD7 Function and Localization

• SETD7 is a histone-lysine N-methyltransferase that specifically monomethylates Lys-4 of histone H3 (H3K4me1), a mark associated with epigenetic transcriptional activation. Related references: PMID:11779497, PMID:11850410, PMID:12540855, PMID:12588998, PMID:16141209
• It plays a central role in the transcriptional activation of target genes such as collagenase and insulin, where it is recruited by transcription factors like IPF1/PDX-1. Related references: PMID:12588998, PMID:16141209
• Beyond histones, SETD7 methylates non-histone substrates including p53/TP53 (at Lys-372), TAF10 (at Lys-189), CGAS (at Lys-491), and GLI3, regulating protein stability, DNA binding, and protein-protein interactions. Related references: PMID:15099517, PMID:15525938, PMID:16415881, PMID:17108971, PMID:27146893
• The protein contains a SET domain and belongs to the SET domain-containing methyltransferase family; it is also known as KMT7, SET7, SET9, or SET7/9.
• SETD7 is localized to the nucleus and associates with chromosomes, consistent with its role in chromatin regulation.
• It is widely expressed across tissues, with expression noted in pancreatic islets, aligning with its role in insulin gene activation.
• Keywords: activator, chromatin regulator, methyltransferase, nucleus, transcription regulation, S-adenosyl-L-methionine.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human SETD7 protein (amino acids 1-366), suggesting the polyclonal antibody may recognize multiple epitopes; consider using appropriate positive and negative controls in your experiments.
• For Western blot (WB), a band at approximately 41 kDa is expected; pre-stained molecular weight markers and loading controls are recommended.
• In immunocytochemistry/immunofluorescence (IF/ICC), anticipate nuclear localization with possible chromosomal association; include DAPI co-staining to confirm subcellular distribution.
• This antibody has been reported to react with human, mouse, and rat samples; when using with other species, sequence homology analysis and pilot experiments are advisable.
• [KO Validated] indicates the antibody's specificity has been confirmed using knockout samples; users may consider including knockdown or knockout controls to verify specificity in their own system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to critical raw materials. This SETD7 polyclonal antibody exemplifies our commitment to supplying well-characterized reagents for chromatin and epigenetics research. Our one-stop platform also delivers matched antibody pairs, ancillary reagents, and bespoke sourcing for unique targets, accelerating your IVD development pipeline.