[KO Validated] Anti-SERPINB5 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P36952

Core Product Specifications and Parameters

Biological Background: SERPINB5 Function and Localization

• Serpin B5, also known as maspin or peptidase inhibitor 5 (PI-5), is a non-inhibitory member of the serine protease inhibitor superfamily encoded by the SERPINB5 gene.
• Unlike classical serpins, maspin does not undergo the strained-to-relaxed (S-to-R) conformational change required for protease inhibition and thus lacks serine protease inhibitory activity.
• Maspin functions as a tumor suppressor, blocking the growth, invasion, and metastatic properties of mammary tumors.
• Subcellular localization is primarily in the secreted extracellular space, consistent with its role in influencing the tumor microenvironment.
• It is specifically expressed in normal mammary epithelial cells, and its downregulation is associated with cancer progression.
• Post-translational modifications include glycosylation; the protein has a molecular weight of approximately 42 kDa.
• Alternative splicing of the SERPINB5 gene produces multiple transcript variants.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal region (aa 21-189) of SERPINB5, which is exposed in the native protein and suitable for generating antibodies that recognize both secreted and intracellular forms.
• For Western blotting, expect a band around 42 kDa; post-translational modifications may cause minor shifts. Use appropriate lysates from human mammary epithelial cell lines (e.g., MCF-7).
• In IHC-P, consider antigen retrieval protocols and test on formalin-fixed paraffin-embedded tissue sections; maspin is cytoplasmic and secreted, so membrane permeabilization may not be required.
• The antibody is predicted to cross-react with mouse SERPINB5 due to sequence homology; validate in mouse tissues if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. Our SERPINB5 polyclonal antibody supports tumor suppressor research and breast cancer diagnostic development. Contact us for validated antibody pairs, bulk ancillary reagents, and customized rare-target sourcing solutions.