[KO Validated] Anti-SEPHS1 Rabbit Polyclonal Antibody - P49903

Core Product Specifications and Parameters

Biological Background: SEPHS1 Function and Localization

• SEPHS1 is a core component of the zincore complex, a heterotetramer that acts as a molecular 'grip' to stabilize transcription factors at DNA-binding sites, thereby controlling gene expression (PubMed:40608935).
  Related references: PMID:40608935
• It specifically binds zinc finger transcription factors, such as ZFP91, ZNF652, ZNF526 and PRDM15, via its arginine clamp, enhancing their DNA-binding stability in a sequence-independent manner (PubMed:40608935).
  Related references: PMID:40608935
• SEPHS1 is also implicated in redox homeostasis (PubMed:31607477), and it may participate in selenocysteine biosynthesis by catalyzing selenophosphate formation, though its in vivo role remains unclear (PubMed:7665581; PubMed:15534230).
  Related references: PMID:31607477; PMID:7665581; PMID:15534230
• Subcellularly, the protein localizes to the nucleus (chromosome), cytoplasm, and cell membrane, with additional presence at the nuclear membrane.
• SEPHS1 undergoes acetylation and alternative splicing, and its expression is cell cycle-regulated, gradually increasing until G2/M phase before declining.
• Keywords from UniProt include 3D-structure, ATP-binding, kinase, magnesium, metal-binding, and selenium, reflecting its enzymatic and regulatory roles.
• The protein is required for cardiac differentiation, as inferred from similarity.
• Disease variants in SEPHS1 have been associated with intellectual disability.

Experimental Guidance and Technical Tips

• The immunogen, covering full-length human SEPHS1 (amino acids 1-392), is expected to provide broad epitope coverage; consider this when designing blocking or competition experiments.
• For Western blot (WB), the predicted molecular weight is ~43 kDa; ensure gel percentage and transfer conditions are optimized for this range.
• In immunofluorescence (IF/ICC), note the protein's nuclear and cytoplasmic localization, and include appropriate controls to distinguish these compartments.
• For immunohistochemistry (IHC-P), validate in tissue samples known to express SEPHS1, as its expression may be regulated during the cell cycle.
• The polyclonal nature of the antibody may recognize multiple epitopes, so lot-to-lot consistency should be verified for quantitative assays like ELISA.
• Cross-reactivity with mouse and rat SEPHS1 is reported; confirm compatibility with the specific sample system of interest.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with seamless access to a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-SEPHS1 polyclonal antibody. Whether you need matched antibody pairs, bulk ancillary reagents, or assistance sourcing rare targets, CamelBio streamlines your procurement from concept to clinic. Explore our antibody catalog to accelerate your zincore complex and transcription factor stability studies.