[KO Validated] Anti-SELENBP1 Rabbit Polyclonal Antibody - Q13228

Core Product Specifications and Parameters

Biological Background: SELENBP1 Function and Localization

• SELENBP1, also known as methanethiol oxidase, 56 kDa selenium-binding protein (SBP56), or SP56, is encoded by the SELENBP1 gene and exists in multiple isoforms due to alternative splicing.
• The primary enzymatic activity of SELENBP1 is the oxidation of methanethiol, a volatile organosulfur compound produced by gut bacteria, highlighting its role in sulfur metabolism. Related references: PMID:29255262
• As a selenium-binding protein, SELENBP1 is thought to participate in the cytoplasmic sensing of reactive xenobiotics, potentially facilitating detoxification processes.
• SELENBP1 may also contribute to intra-Golgi protein transport, suggesting a role in cellular trafficking pathways.
• Subcellular localization studies indicate that SELENBP1 is present in the nucleus, cytoplasm, cytosol, and membrane compartments, reflecting its multifunctional nature.
• Post-translational modifications include acetylation and phosphorylation, and the protein is classified as an oxidoreductase with selenium-binding capacity, underlining its regulatory and catalytic versatility.
• SELENBP1 is widely expressed with high levels in liver, lung, colon, prostate, kidney, and pancreas. In the brain, it is found in both neurons and glia. Notably, its expression is down-regulated in several cancers (lung adenocarcinoma, colorectal carcinoma, ovarian cancer) and up-regulated in brain and blood from schizophrenia patients, implicating it in disease pathology.

Experimental Guidance and Technical Tips

• This antibody has been knockout validated, providing enhanced confidence for detecting endogenous SELENBP1.
• For Western blot, expect a band at approximately 52 kDa; verify detection using positive controls such as liver or colon lysates.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions to visualize nuclear and cytoplasmic localization.
• For ELISA, the immunogen corresponds to the C-terminal region (a.a. 243-472); consider this epitope coverage when designing assays.

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